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Molecular identification of Na+–H+ exchanger isoforms (NHE2) in the gills of the euryhaline teleost Fundulus heteroclitus

Authors

  • S. L. Edwards,

    Corresponding author
    1. Department of Biology, Appalachian State University, Boone, NC 28608, U.S.A.
    2. Mount Desert Island Biological Laboratory, Salisbury Cove, ME 04672, U.S.A.
      Tel.: +1 828 262 2679; fax: +1 828 262 2127; email: edwardssl@appstate.edu
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  • J. C. Weakley,

    1. Department of Biology, Georgia Southern University, Statesboro, GA 30460, U.S.A.
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  • A. W. Diamanduros,

    1. Department of Biology, Georgia Southern University, Statesboro, GA 30460, U.S.A.
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  • J. B. Claiborne

    1. Mount Desert Island Biological Laboratory, Salisbury Cove, ME 04672, U.S.A.
    2. Department of Biology, Georgia Southern University, Statesboro, GA 30460, U.S.A.
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Tel.: +1 828 262 2679; fax: +1 828 262 2127; email: edwardssl@appstate.edu

Abstract

In the current study, reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR were used to clone full-length putative Na+–H+ exchanger isoforms (NHE2a) cDNA from the gills of Fundulus heteroclitus. The 2480 bp cDNA includes a coding region for a protein that shows a 57% amino acid homology to rabbit NHE2. These sequences allowed data mining of available fish genome data, which revealed at least three NHE2 subtypes in some teleost species.

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