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Keywords:

  • cardiac regeneration;
  • fish heart;
  • immunohistochemistry;
  • Oncorhynchus mykiss;
  • primary cell culture;
  • rainbow trout

In primary cell preparations from larvae of rainbow trout Oncorhynchus mykiss, the formation of autonomously contracting cell aggregates was observed after 7 days. These contracting elements could be propagated and some aggregates were maintained over a period of 35 days. Electron microscopical and immunocytochemical examination revealed the presence of cardiomyocytes.