Discrimination of two picarel species Spicara flexuosa and Spicara maena (Pisces: Centracanthidae) based on mitochondrial DNA sequences

Authors

  • A. Imsiridou,

    1. Alexander Technological Educational Institute of Thessaloniki, Department of Aquaculture & Fisheries Technology, P. O. Box 157, GR-63200, Nea Moudania, Greece
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  • G. Minos,

    Corresponding author
    1. Alexander Technological Educational Institute of Thessaloniki, Department of Aquaculture & Fisheries Technology, P. O. Box 157, GR-63200, Nea Moudania, Greece
      Tel.: +30 23730 65313; email: gminos@otenet.gr
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  • A. Gakopoulou,

    1. Alexander Technological Educational Institute of Thessaloniki, Department of Aquaculture & Fisheries Technology, P. O. Box 157, GR-63200, Nea Moudania, Greece
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  • V. Katsares,

    1. Alexander Technological Educational Institute of Thessaloniki, Department of Aquaculture & Fisheries Technology, P. O. Box 157, GR-63200, Nea Moudania, Greece
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  • T. Karidas,

    1. Alexander Technological Educational Institute of Thessaloniki, Department of Aquaculture & Fisheries Technology, P. O. Box 157, GR-63200, Nea Moudania, Greece
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  • G. Katselis

    1. Technological Educational Institute of Messolonghi, Department of Aquaculture & Fisheries Management, GR-30200, Messolonghi, Greece
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Tel.: +30 23730 65313; email: gminos@otenet.gr

Abstract

A DNA methodology based on PCR and sequencing analysis of the mtDNA 16S rRNA gene was developed for the discrimination of picarel Spicara flexuosa and blotched picarel Spicara maena (Pisces: Centracanthidae). The molecular results indicated that there is a clear discrimination between the two species, as all the individuals of S. flexuosa revealed the same 16S rDNA haplotype while the S. maena haplotype differs in 15 distinct nucleotides.

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