Cloning of Arctic lamprey Lethenteron camtschaticum cd9 with roles in the immune response

Authors

  • F. Wu,

    1. College of Life Science, Liaoning Normal University, Dalian 116029, China
    2. Institute of Marine Genomics & Proteomics, Liaoning Normal University, Dalian 116029, China
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  • P. Su,

    1. College of Life Science, Liaoning Normal University, Dalian 116029, China
    2. Institute of Marine Genomics & Proteomics, Liaoning Normal University, Dalian 116029, China
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  • L. Chen,

    1. College of Life Science, Liaoning Normal University, Dalian 116029, China
    2. Institute of Marine Genomics & Proteomics, Liaoning Normal University, Dalian 116029, China
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  • M. Li,

    1. College of Life Science, Liaoning Normal University, Dalian 116029, China
    2. Institute of Marine Genomics & Proteomics, Liaoning Normal University, Dalian 116029, China
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  • X. Liu,

    1. College of Life Science, Liaoning Normal University, Dalian 116029, China
    2. Institute of Marine Genomics & Proteomics, Liaoning Normal University, Dalian 116029, China
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  • Q. Li

    Corresponding author
    1. College of Life Science, Liaoning Normal University, Dalian 116029, China
    2. Institute of Marine Genomics & Proteomics, Liaoning Normal University, Dalian 116029, China
      Tel.: +86 411 82156555; email: liqw@263.net
    Search for more papers by this author

Tel.: +86 411 82156555; email: liqw@263.net

Abstract

In this study, the cd9 gene, a member of the tetraspanin superfamily and involved in various cellular processes, was cloned from Lethenteron camtschaticum. Both real-time PCR and immunohistochemical assays showed broad distribution of cd9 in various L. camtschaticum tissues. In addition, expression levels of Cd9 mRNA were up-regulated in the liver and heart after stimulation by lipopolysaccharide. Flow cytometric analyses demonstrated that cd9 was detected on the leukocytes and that the expression level was higher on granulocytes than on lymphocytes, which implied that cd9 was mainly involved in innate immunity.

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