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APPENDIX S1.

FIGURE SI. Deduced amino acid sequence alignment of Lates calcarifer heat shock protein 90 alpha and beta (hsp90AA & hsp90AB). The positions of primers used in this work are marked by a line over the appropriate sequence position and accompanied by the primer name. The conserved -variable regions are separated by ‖ with the corresponding name above the alignment. The C-terminal MEEVD sequence characteristic of cytosolic Hsp90 members is shaded. hsp90AA is used as the reference sequence and only differences are shown for Hsp90AB; dots indicate identical amino acids and dashes indicate gaps. ▾, functionally important residues (see text).

FIGURE SII.

FIGURE SIII.

FIGURE SIV.

TABLE SI. Universal primers sequences used to isolate L. calcarifer specific sequences. Gene names are given in the abbreviated form; hsp (heat-shock protein), cisy (citrate synthase), cco II (cytochrome oxidase C subunit two), ldh (lactate dehydrogenase), igf1 (insulin like growth factor 1) and mstn-1 (myostatin 1).

TABLE SII. GeneRacer species compatible external and internal primers used to obtain full length Lates calcarifer gene sequences. Gene names are given in the abbreviated form; hsp (heat-shock protein), cisy (citrate synthase), cco II (cytochrome oxidase C subunit two), ldh (lactate dehydrogenase), igf-1 (insulin-like growth factor 1) and mstn-1 (myostatin 1).

TABLE SIII. Lates calcarifer qrt-PCR primers used to examine the expression of genes of interest in the current study. Gene names are given in the abbreviated form; hsp (heat-shock protein), cisy (citrate synthase), cco II (cytochrome oxidase C subunit two), ldh (lactate dehydrogenase), igf-1 (insulin-like growth factor 1) and mstn-1 (myostatin 1). E-1(%) gives the reaction efficiency as a percentage and is calculated using a standard curve with the equation E = 10(−1/slope).

FilenameFormatSizeDescription
JFB_3310_sm_fs1.eps706KSupporting info item
JFB_3310_sm_fs2.eps640KSupporting info item
JFB_3310_sm_fs3.eps303KSupporting info item
JFB_3310_sm_fs4.eps902KSupporting info item
JFB_3310_sm_as1.docx20KSupporting info item
JFB_3310_sm_ts1_ts2_ts3.docx16KSupporting info item

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