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Isolation of novel microsatellite loci in the black goby Gobius niger and cross-amplification in other gobiid species (Perciformes, Gobiidae)

Authors

  • P. Ruggeri,

    1. Dipartimento di Scienze della Vita e dell’Ambiente, Università Politecnica delle Marche, Ancona 60131, Italy
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  • A. Splendiani,

    1. Dipartimento di Scienze della Vita e dell’Ambiente, Università Politecnica delle Marche, Ancona 60131, Italy
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  • M. Giovannotti,

    Corresponding author
    1. Dipartimento di Scienze della Vita e dell’Ambiente, Università Politecnica delle Marche, Ancona 60131, Italy
      Tel.: +39 71 2204134; email: m.giovannotti@univpm.it
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  • P. Nisi Cerioni,

    1. Dipartimento di Scienze della Vita e dell’Ambiente, Università Politecnica delle Marche, Ancona 60131, Italy
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  • V. Caputo

    1. Dipartimento di Scienze della Vita e dell’Ambiente, Università Politecnica delle Marche, Ancona 60131, Italy
    2. Consiglio Nazionale delle Ricerche, Istituto di Scienze Marine Sezione Pesca Marittima, Largo Fiera della Pesca, 60125 Ancona, Italy
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Tel.: +39 71 2204134; email: m.giovannotti@univpm.it

Abstract

Twelve microsatellite loci were isolated from and characterized for the black goby Gobius niger. These loci were tested on a total of 48 individuals from two geographically distant locations (Orbetello and Cattolica) and the number of alleles ranged from two to 18, with expected (He) and observed (Ho) heterozygosities ranging from 0·042 to 0·941 and from 0·042 to 0·917, respectively. The loci described were used to cross-amplify other gobiid species belonging to Gobius, Zosterisessor, Lesueurigobius and Aphia.

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