Water chemistry in the microenvironment of rainbow trout Oncorhynchus mykiss embryos is affected by development, the egg capsule and crowding


Tel.: +1 519 824 4120 ext. 52719; email: hdhiyebi@uoguelph.ca


The hypothesis tested was that embryonic metabolism affects the water chemistry in the boundary layer. In addition, embryo crowding would further compound the metabolic effect on the water chemistry in the boundary layer. As development progressed, the magnitude of the boundary layer gradients for O2 and pH, but not for NHinline image, increased. The presence of the egg capsule hindered the diffusion of O2 into and H+ and NHinline image out of the embryo. The magnitude of the O2, pH and NHinline image boundary layer gradient was significantly increased when embryos were surrounded by either sham embryos or live embryos. The majority of this crowding effect on embryo boundary layers was due to changes in water flow rather than due to metabolism directly. These results clearly show that the microenvironment adjacent to the developing rainbow trout Oncorhynchus mykiss embryo becomes more stagnant as development progresses in the presence of the egg capsule and is further intensified with embryo crowding.