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Appendix S1.Galictis specimens used in the morphological, genetic and geographic analyses listed per species per institution.

Appendix S2. Results of the t-test comparison for sexual size dimorphism within Galictis cuja and within G. vittata.

Appendix S3. GenBank accession numbers for previously published mitochondrial and nuclear sequences of two mustelids used as outgroups in the current study (Ictonyx striatus and Poecilogale albinucha).

Appendix S4. Classification coefficients from the classification analyses for each Galictis species (G. cuja and G. vittata) extracted from the two-group and multiple-group discriminant function analysis (DFA).

Appendix S5. A. Table depicting the variable sites found within and between Galictis cuja and G. vittata in 12 nuclear gene segments. B. Haplotype networks derived from the nucleotide information described in A for 11 of the 12 nuclear segments.

Appendix S6. Geographic distribution map of Galictis vittata and G. cuja throughout the Neotropical region based on skulls, skins and DNA samples.

Appendix S7. Results from the multiple-group Discriminant Function Analysis (DFA) applying the stepwise method.

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