Expression of inducible nitric oxide synthase and cyclooxygenase-2 mRNA in brain damage induced by lipopolysaccharide and intermittent hypoxia–ischemia in neonatal rats
Article first published online: 10 MAR 2005
Journal of Obstetrics and Gynaecology Research
Volume 31, Issue 2, pages 185–191, April 2005
How to Cite
Yang, L., Sameshima, H., Yamaguchi, M. and Ikenoue, T. (2005), Expression of inducible nitric oxide synthase and cyclooxygenase-2 mRNA in brain damage induced by lipopolysaccharide and intermittent hypoxia–ischemia in neonatal rats. Journal of Obstetrics and Gynaecology Research, 31: 185–191. doi: 10.1111/j.1341-8076.2005.00266.x
- Issue published online: 10 MAR 2005
- Article first published online: 10 MAR 2005
- Received: September 14 2004. Accepted: December 8 2004.
- inducible nitric oxide synthase;
- intermittent hypoxia–ischemia;
- neonatal rat
Aim: The purpose of the present study was to examine the effect of lipopolysaccharide (LPS) and intermittent hypoxia–ischemia (HI) on brain damage in neonatal rats.
Methods: Seven-day-old Wistar rats were injected with saline or LPS (1 mg/kg), and then underwent left common carotid artery ligation followed by a repetitive 8% hypoxia (2.0–4.5 min) at 10-min intervals 10 times. The rats were divided into three groups: LPS with HI (LPS/HI, n = 46), saline with HI (HI alone, n = 42) and LPS alone (n = 16). Seven days later, brains were assessed for neuronal damage and inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) mRNA expression.
Results: Neuronal damage in the ligated side was significantly higher in LPS/HI than the other two groups (P < 0.01). The expression of iNOS and COX-2 mRNA was observed in the affected brain in LPS/HI, which corresponded well to histologic neuronal loss.
Conclusions: LPS enhanced intermittent HI brain damage in immature animals. The expression of iNOS and COX-2 mRNA is considered to be associated with perinatal brain injury processes.