Screening of marine algae for potential tyrosinase inhibitor: Those inhibitors reduced tyrosinase activity and melanin synthesis in zebrafish
Article first published online: 6 SEP 2010
© 2010 Japanese Dermatological Association
The Journal of Dermatology
Volume 38, Issue 4, pages 354–363, April 2011
How to Cite
CHA, S.-H., KO, S.-C., KIM, D. and JEON, Y.-J. (2011), Screening of marine algae for potential tyrosinase inhibitor: Those inhibitors reduced tyrosinase activity and melanin synthesis in zebrafish. The Journal of Dermatology, 38: 354–363. doi: 10.1111/j.1346-8138.2010.00983.x
- Issue published online: 22 MAR 2011
- Article first published online: 6 SEP 2010
- Received 21 June 2009; accepted 11 May 2010.
- marine algae;
- melanin synthesis inhibitor;
- tyrosinase inhibitor;
In order to find new anti-browning and whitening agents in this study, we investigated 43 indigenous marine algae for tyrosinase inhibitory activity. The extracts from Endarachne binghamiae, Schizymenia dubyi, Ecklonia cava (EC) and Sargassum silquastrum (SS) evidenced potent tyrosinase inhibitory activity similar to that of positive control, kojic acid. Among those marine algae, EC and SS are distributed abundantly on Jeju Island. Therefore, we selected those two species for further studies. Our results evidenced that both species reduced cellular melanin synthesis and tyrosinase activity. On the other hand, we utilized zebrafish as an alternative in vivo model. All the tested samples evidenced excellent inhibitory effects on the pigmentation of zebrafish, most likely due to their potential tyrosinase inhibitory activity. In simultaneous in vivo toxicity tests, no toxicity was observed in either algal species, on the other hand, toxicity was observed in positive controls. These results provided that EC and SS extract could be used as an ingredient for whiting cosmetics and that zebrafish is an alternative in vivo model.