• animal experiment;
  • influenza;
  • rapid diagnosis


  1. Top of page
  2. Abstract
  3. References

The ESPLINE® INFLUENZA A&B-N kit was evaluated for its applicability to the rapid diagnosis of influenza in chickens and pigs. The kit specifically detected viral antigens in tracheal swabs and tissue homogenates of the trachea, liver, spleen, and colon of chickens inoculated with a highly pathogenic avian influenza virus strain, A/chicken/Yamaguchi/7/04 (H5N1), at 48 hr post-inoculation (p.i.) as well as in the tracheal and cloacal swabs and tissue homogenates of dead chickens. For those infected with a low pathogenic strain, A/chicken/aq-Y-55/01 (H9N2), antigens were detected only in the samples from tracheal swabs and organs 1–4 days p.i. The kit also detected viral antigens in the nasal swabs of miniature pigs infected with swine and avian influenza viruses. The kit was found to be sensitive and specific enough for the rapid diagnosis of infections of influenza A virus in chickens and pigs.


avian paramyxovirus


biosafety containment level III




50% egg infectious dose




highly pathogenic avian influenza


low pathogenic avian influenza


Madin-Darby canine kidney cell






reverse transcription polymerase chain reaction


specific pathogen-free




50% tissue infectious dose.


  1. Top of page
  2. Abstract
  3. References
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