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Keywords:

  • animal experiment;
  • influenza;
  • rapid diagnosis

Abstract

  1. Top of page
  2. Abstract
  3. References

The ESPLINE® INFLUENZA A&B-N kit was evaluated for its applicability to the rapid diagnosis of influenza in chickens and pigs. The kit specifically detected viral antigens in tracheal swabs and tissue homogenates of the trachea, liver, spleen, and colon of chickens inoculated with a highly pathogenic avian influenza virus strain, A/chicken/Yamaguchi/7/04 (H5N1), at 48 hr post-inoculation (p.i.) as well as in the tracheal and cloacal swabs and tissue homogenates of dead chickens. For those infected with a low pathogenic strain, A/chicken/aq-Y-55/01 (H9N2), antigens were detected only in the samples from tracheal swabs and organs 1–4 days p.i. The kit also detected viral antigens in the nasal swabs of miniature pigs infected with swine and avian influenza viruses. The kit was found to be sensitive and specific enough for the rapid diagnosis of infections of influenza A virus in chickens and pigs.

Abbreviations
APMV

avian paramyxovirus

BSL 3

biosafety containment level III

Dk

duck

EID50

50% egg infectious dose

HA

haemagglutinin

HPAI

highly pathogenic avian influenza

LPAI

low pathogenic avian influenza

MDCK

Madin-Darby canine kidney cell

NP

nucleoprotein

p.i.

post-inoculation

RT-PCR

reverse transcription polymerase chain reaction

SPF

specific pathogen-free

Sw

swine

TCID50

50% tissue infectious dose.

References

  1. Top of page
  2. Abstract
  3. References
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