Infection of primary cultures of murine splenic and thymic cells with coxsackievirus B4

Authors

  • Hela Jaïdane,

    1. Virology Laboratory/UPRES EA3610, Faculty of Medicine, University of Lille 2, Bâtiment Paul Boulanger, CHRU Lille, 59037 Lille, France
    2. Laboratory Lab-MDT-01, Unit of Pathogenesis and Viral Virulence, Faculty of Pharmacy of Monastir, Avenue Avicenne 5000 Monastir, Tunisia
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  • Jawhar Gharbi,

    1. Laboratory Lab-MDT-01, Unit of Pathogenesis and Viral Virulence, Faculty of Pharmacy of Monastir, Avenue Avicenne 5000 Monastir, Tunisia
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  • Pierre-Emmanuel Lobert,

    1. Virology Laboratory/UPRES EA3610, Faculty of Medicine, University of Lille 2, Bâtiment Paul Boulanger, CHRU Lille, 59037 Lille, France
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  • Delphine Caloone,

    1. Virology Laboratory/UPRES EA3610, Faculty of Medicine, University of Lille 2, Bâtiment Paul Boulanger, CHRU Lille, 59037 Lille, France
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  • Bernadette Lucas,

    1. Virology Laboratory/UPRES EA3610, Faculty of Medicine, University of Lille 2, Bâtiment Paul Boulanger, CHRU Lille, 59037 Lille, France
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  • Famara Sané,

    1. Virology Laboratory/UPRES EA3610, Faculty of Medicine, University of Lille 2, Bâtiment Paul Boulanger, CHRU Lille, 59037 Lille, France
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  • Thierry Idziorek,

    1. Inserm U459, Faculty of Medicine
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  • Marie-Bénédicte Romond,

    1. Laboratory of Bacteriology, Faculty of Pharmaceutical and Biological Sciences, 59037 Lille, France
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  • Mahjoub Aouni,

    1. Laboratory Lab-MDT-01, Unit of Pathogenesis and Viral Virulence, Faculty of Pharmacy of Monastir, Avenue Avicenne 5000 Monastir, Tunisia
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  • Didier Hober

    1. Virology Laboratory/UPRES EA3610, Faculty of Medicine, University of Lille 2, Bâtiment Paul Boulanger, CHRU Lille, 59037 Lille, France
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Correspondence
Didier Hober, Laboratoire de Virologie/UPRES EA3610, Bâtiment Paul Boulanger, CHRU Lille, 59037 Lille, France. Tel: +33 3 20 44 69 30; fax: +33 3 20 44 52 81; email: dhober@chru-lille.fr

ABSTRACT

Infection of primary cultures of total splenic and thymic cells from BALB/c and C3H/HeN mice with CVB4 E2 and JVB strains has been investigated. The presence of positive-strand viral RNA within cells was determined by semi-nested RT-PCR, and viral replication was attested by detection of intracellular negative-strand viral RNA and by release of infectious particles in culture supernatants. Viral replication occurred with both CVB4 strains to an extent dependent on the genetic background of the host. No interferon-α production was detected in the supernatants of CVB4-infected cultures using biological titration. Together these results suggest that infection of splenic and thymic cells can play a role in virus dissemination, and therefore in the pathophysiology of CVB4 infections.

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