Rapid culture-free identification and molecular typing of Shiga toxin-producing Escherichia coli by PCR-RFLP

Authors


Correspondence
Shinji Yamasaki, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-Cho, Naka-ku, Sakai, Osaka 599-8531, Japan. Tel & fax: +81 72 254 9515; email: shinji@vet.osakafu-u.ac.jp

ABSTRACT

Using culture-independent technology, PCR-RFLP were used to identify and type STEC in the stool of a patient with HUS. Fecal PCR-RFLP patterns were identical to those of the STEC O157:H7 isolated from the patient.

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