These authors contributed equally to this work.
Screen of multifunctional monoclonal antibodies against hepatitis B core virus-like particles
Article first published online: 16 MAR 2009
© 2009 The Societies and Blackwell Publishing Asia Pty Ltd
Microbiology and Immunology
Volume 53, Issue 6, pages 340–348, June 2009
How to Cite
Sun, C., Ding, F.-X., Wang, F., He, X.-W., He, Y., Li, Z.-S. and Sun, S.-H. (2009), Screen of multifunctional monoclonal antibodies against hepatitis B core virus-like particles. Microbiology and Immunology, 53: 340–348. doi: 10.1111/j.1348-0421.2009.00135.x
- Issue published online: 29 MAY 2009
- Article first published online: 16 MAR 2009
- Received 3 December 2008; revised 11 February 2009; accepted 2 March 2009.
- conformational epitope;
- epitope presentation system;
- Hepatitis B virus core antigen;
- virus-like particle
HBc-VLP can be used in an epitope presentation system to carry foreign epitopes and mimic live virus in order to study viral particle uptake, virion-mediated activation and antigen presentation by dendritic cells. In this study, a multifunctional mAb was produced using a novel research strategy. A truncated HBc-VLP bone vector with a special conformation was used as an immunogen and the target hybridoma cell lines were screened by a series of tests; including ELISA, Western blot, and cellular immunofluorescence based on the epitope presentation system. The screened monoclonal antibody was used to identify the HBc-VLP vector, a fusion HBc-VLP vaccine, and intracellular HBV capsids. The new strategy facilitated acquisition of the desired mAbs and will serve as a reference for other VLP-related research.