These authors contributed equally to this study.
Evolution of HIV-1 in a patient population failing multiple-drug therapy
Article first published online: 16 JUN 2009
© 2009 The Societies and Blackwell Publishing Asia Pty Ltd
Microbiology and Immunology
Volume 53, Issue 9, pages 535–539, September 2009
How to Cite
Hong, S., Cao, J. and Tu, Y.-t. (2009), Evolution of HIV-1 in a patient population failing multiple-drug therapy. Microbiology and Immunology, 53: 535–539. doi: 10.1111/j.1348-0421.2009.00153.x
- Issue published online: 24 AUG 2009
- Article first published online: 16 JUN 2009
- Received 26 March 2009; revised 20 May 2009; accepted 01 June 2009.
- multiple-drug resistance
To understand the evolutionary pathway of the multi-drug-resistant virus HIV-1 under drug-induced selection pressure, plasma from seven patients from baseline to different intervals post-treatment failure were used in RT-PCR protocols. Multiple clones were sequenced for each time point. Drug-resistant mutations were detected in five patients. Phylogenetic analysis showed that at different time points, viral sequences clustered separately and formed independent lineages. Genetic diversity decreased from 1.59 to 0.55, whereas non-synonymous/synonymous mutation ratios increased from 0.067 to 0.118, respectively. These data suggest that the virus population changed dynamically and clustered in a time point-specific manner whereas genetic diversity decreased consistently.