Evolution of HIV-1 in a patient population failing multiple-drug therapy

Authors

  • Shaolin Hong,

    1. Duke Comprehensive Cancer Center, Duke University Medical Center, Durham, North Carolina 27710, USA
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    • These authors contributed equally to this study.

  • Jingjiang Cao,

    1. Department of Dermatology and Venereology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
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    • These authors contributed equally to this study.

  • Ya-ting Tu

    1. Department of Dermatology and Venereology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
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Correspondence
Ya-ting Tu, Department of Dermatology and Venereology, Union Hospital, Tongji Medical College, Wuhan 430022, China.
Tel: +86 27 85733882; fax: +86 27 83693500; email: yatingtu@yahoo.com.cn

ABSTRACT

To understand the evolutionary pathway of the multi-drug-resistant virus HIV-1 under drug-induced selection pressure, plasma from seven patients from baseline to different intervals post-treatment failure were used in RT-PCR protocols. Multiple clones were sequenced for each time point. Drug-resistant mutations were detected in five patients. Phylogenetic analysis showed that at different time points, viral sequences clustered separately and formed independent lineages. Genetic diversity decreased from 1.59 to 0.55, whereas non-synonymous/synonymous mutation ratios increased from 0.067 to 0.118, respectively. These data suggest that the virus population changed dynamically and clustered in a time point-specific manner whereas genetic diversity decreased consistently.

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