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Characterization of integrons and antimicrobial resistance genes in clinical isolates of Gram-negative bacteria from Palestinian hospitals

  1. Amjad I. A. Hussein1,
  2. Ashraf M. Ahmed3,
  3. Maiko Sato2,
  4. Tadashi Shimamoto2

Article first published online: 11 AUG 2009

DOI: 10.1111/j.1348-0421.2009.00168.x

Issue

Microbiology and Immunology

Microbiology and Immunology

Volume 53, Issue 11, pages 595–602, November 2009

Additional Information

How to Cite

Hussein, A. I. A., Ahmed, A. M., Sato, M. and Shimamoto, T. (2009), Characterization of integrons and antimicrobial resistance genes in clinical isolates of Gram-negative bacteria from Palestinian hospitals. Microbiology and Immunology, 53: 595–602. doi: 10.1111/j.1348-0421.2009.00168.x

Author Information

  1. 1

    Graduate School of Medical Science, Kanazawa University, 13-1 Takaramachi, Kanazawa 920-8640

  2. 2

    Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan

  3. 3

    Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafr El-Sheikh University, Kafr El-Sheikh 33516, Egypt

*Correspondence Tadashi Shimamoto, Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan. Tel/fax: +81 82 424 7897; email: tadashis@hiroshima-u.ac.jp

Publication History

  1. Issue published online: 23 OCT 2009
  2. Article first published online: 11 AUG 2009
  3. Received 29 March 2009; revised 1 July 2009; accepted 3 July 2009.

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Keywords:

  • β-lactamases;
  • Middle East;
  • multidrug resistance;
  • qnr

ABSTRACT

Sixty Gram-negative bacterial isolates were collected from Palestinian hospitals in 2006. Thirty-two (53.3%) isolates showed multidrug resistance phenotypes. PCR and DNA sequencing were used to characterize integrons and antimicrobial resistance genes. PCR screening showed that 19 (31.7%) and five (8.3%) isolates were positive for class 1 and class 2 integrons, respectively. DNA-sequencing results for the captured antimicrobial resistance gene cassettes within class 1 integrons identified the following genes: dihydrofolate reductases, dfrA1, dfrA5, dfrA7, dfrA12, dfrA17 and dfrA25; aminoglycoside adenyltransferases, aadA1, aadA2, aadA5, aadA12 and aadB; aminoglycoside acetyltransferase, aac(6′)-Ib; and chloramphenicol resistance gene, cmlA1. ESBL were identified in 25 (41.7%) isolates. The identified ESBL were blaCTX-M-15, blaCTX-M-56, blaOXA-1, blaSHV-1, blaSHV-12, blaSHV-32 and blaTEM-1 genes. Moreover, we characterized the plasmid-mediated quinolone resistance genes, aac(6′)-Ib-cr and qnrB2, which were detected in seven (11.7%) and two (3.3%) isolates, respectively. In this study various types of antibiotic resistance genes have been identified in Gram-negative bacteria from Palestinian hospitals, many of which are reported in the Middle East area for the first time.

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