Bifidobacterium suppresses IgE-mediated degranulation of rat basophilic leukemia (RBL-2H3) cells

Authors

  • Gaku Harata,

    1. Technical Research Laboratory, Takanashi Milk Products Co. Ltd., 5 Honjyuku-cho, Asahi-ku, Yokohama, Kanagawa 241-0023
    2. Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan
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  • Fang He,

    1. Technical Research Laboratory, Takanashi Milk Products Co. Ltd., 5 Honjyuku-cho, Asahi-ku, Yokohama, Kanagawa 241-0023
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  • Kyoko Takahashi,

    1. Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan
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  • Akira Hosono,

    1. Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan
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  • Manabu Kawase,

    1. Technical Research Laboratory, Takanashi Milk Products Co. Ltd., 5 Honjyuku-cho, Asahi-ku, Yokohama, Kanagawa 241-0023
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  • Akira Kubota,

    1. Technical Research Laboratory, Takanashi Milk Products Co. Ltd., 5 Honjyuku-cho, Asahi-ku, Yokohama, Kanagawa 241-0023
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  • Masaru Hiramatsu,

    1. Technical Research Laboratory, Takanashi Milk Products Co. Ltd., 5 Honjyuku-cho, Asahi-ku, Yokohama, Kanagawa 241-0023
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  • Shuichi Kaminogawa

    1. Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan
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Correspondence
Fang He, Technical Research Laboratory, Takanashi Milk Products Co. Ltd., 5 Honjyuku-cho, Asahi-ku, Yokohama, Kanagawa 241-0023, Japan. Tel: +81 45 367 6645; fax: +81 45 364 2160; email: he-fang@takanashi-milk.co.jp

ABSTRACT

Sixteen heat-killed bifidobacteria isolated from human intestine and a probiotic strain Lactobacillus GG were tested for their ability to influence IgE-mediated degranulation of rat basophilic leukemia (RBL-2H3) cells in vitro. The bifidobacteria suppressed IgE-mediated degranulation of RBL-2H3 cells by 1.6–56.4% in a strain-dependent manner. Bifidobacteria from healthy infants expressed high inhibitory effects on IgE-mediated degranulation (41–55%), while those from allergic infants varied greatly in their effects against degranulation. Bifidobacteria taxonomically identified as Bifidobacterium bifidum exhibited much stronger inhibitory effects against IgE-mediated degranulation than those taxonomically identified as B. adolescentis (P < 0.05).These results indicate that the intestinal bifidobacteria might be one of factors influencing IgE-mediated allergic responses.

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