Low endotoxic activity of lipopolysaccharides isolated from Bradyrhizobium, Mesorhizobium, and Azospirillum strains
Version of Record online: 23 NOV 2010
© 2010 The Societies and Blackwell Publishing Asia Pty Ltd
Microbiology and Immunology
Volume 54, Issue 12, pages 717–725, December 2010
How to Cite
Komaniecka, I., Zdzisinska, B., Kandefer-Szerszen, M. and Choma, A. (2010), Low endotoxic activity of lipopolysaccharides isolated from Bradyrhizobium, Mesorhizobium, and Azospirillum strains. Microbiology and Immunology, 54: 717–725. doi: 10.1111/j.1348-0421.2010.00269.x
- Issue online: 23 NOV 2010
- Version of Record online: 23 NOV 2010
- Accepted manuscript online: 16 SEP 2010 03:57AM EST
- Received 5 July 2010; revised 24 August 2010; accepted 5 September 2010.
The endotoxic activities of lipopolysaccharides (LPS) isolated from different strains of rhizobia and rhizobacteria (Bradyrhizobium, Mesorhizobium, and Azospirillum) were compared to those of Salmonella enterica sv. Typhimurium LPS. The biological activity of all the examined preparations, measured as Limulus lysate gelation, production of tumor necrosis factor (TNF), interleukin-1β (IL-1β), and interleukin-6 (IL-6), and nitrogen oxide (NO) induction in human myelomonocytic cells (line THP-1), was considerably lower than that of the reference enterobacterial endotoxin. Among the rhizobial lipopolysaccharides, the activities of Mesorhizobium huakuii and Azospirillum lipoferum LPSs were higher than those of the LPS preparations from five strains of Bradyrhizobium. The weak endotoxic activity of the examined preparations was correlated with differences in lipid A structure compared to Salmonella.