These authors contributed equally to this work.
A combination of methods to evaluate biofilm production may help to determine the clinical relevance of Staphylococcus in blood cultures
Article first published online: 22 DEC 2010
© 2010 The Societies and Blackwell Publishing Asia Pty Ltd
Microbiology and Immunology
Volume 55, Issue 1, pages 28–33, January 2011
How to Cite
Iorio, N. L. P., Lopes, A. P. d. C. N., Schuenck, R. P., Barcellos, A. G., Olendzki, A. N., Lopez, G. L. and dos Santos, K. R. N. (2011), A combination of methods to evaluate biofilm production may help to determine the clinical relevance of Staphylococcus in blood cultures. Microbiology and Immunology, 55: 28–33. doi: 10.1111/j.1348-0421.2010.00288.x
- Issue published online: 22 DEC 2010
- Article first published online: 22 DEC 2010
- Accepted manuscript online: 23 NOV 2010 12:20AM EST
- Received 11 June 2010; revised 7 October 2010; accepted 21 October 2010.
- biofilm detection methods;
Staphylococcus is the most prevalent pathogen causing bacteremia and many of its isolates possess the ability to form biofilm. In this study Staphylococcus isolates from the blood of patients with bacteremia were analyzed by two biofilm detection phenotypic methods: Congo red agar (CRA) and microtiter-plate adherence (MPA) in relation to the presence of ica genes, detected by PCR. Their oxacillin susceptibility was also evaluated. Among 127 isolates evaluated, 47 were S. aureus and 80 were coagulase negative staphylococci (CNS). Seventy-four (58.3%) isolates were mecA gene positive (27.7%S. aureus and 76.3% CNS isolates). Among the 40 S. aureus isolates which were positive for the ica genes, 25 (62.5%) were positive in MPA and 27 (67.5%) in CRA, whereas both methods combined detected 34 (85%) isolates as biofilm producers. Among 12 S. epidermidis isolates carrying ica genes, 8 were positive in MPA and 5 in CRA. The combination of CRA and MPA methods provided a better prediction of the presence of ica genes in S. aureus isolates than did either method alone.