Uptake dynamics of scrapie agent in the intestinal villous epithelium of suckling and weanling Syrian hamsters

Authors

  • Jyuri Kono,

    1. Laboratory of Biomedical Science, Department of Veterinary Medicine, College of Biosource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880
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  • Ryoko Toyoshima,

    1. Laboratory of Biomedical Science, Department of Veterinary Medicine, College of Biosource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880
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  • Tomoko Iseki,

    1. Laboratory of Biomedical Science, Department of Veterinary Medicine, College of Biosource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880
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  • Yukita Sato,

    1. Laboratory of Biomedical Science, Department of Veterinary Medicine, College of Biosource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880
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  • Atushi Umematu,

    1. Laboratory of Biomedical Science, Department of Veterinary Medicine, College of Biosource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880
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  • Misuzu Okano,

    1. Laboratory of Biomedical Science, Department of Veterinary Medicine, College of Biosource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880
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  • Akiko Tashiro,

    1. Laboratory of Biomedical Science, Department of Veterinary Medicine, College of Biosource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880
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  • Yasuhisa Ano,

    1. Central Laboratories for Frontier Technology, Kirin Holdings, 1-13-5 Fukuura Kanzawa-ku, Yokohama, Kanagawa 236-0004
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  • Tetsutaro Sata,

    1. National Institute of Infectious Diseases,1-23-1 Toyama, Shinjuku, Tokyo 162-8640
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  • Takashi Yokoyama,

    1. Prion Disease Research Center, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856
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  • Takashi Onodera,

    1. Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
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  • Masayoshi Yukawa

    1. Laboratory of Biomedical Science, Department of Veterinary Medicine, College of Biosource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880
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Jyuri Kono, Laboratory of Biomedical Science, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
Tel and fax: +81 466 84 3445; email: gr16510t@st.brs.nihon-u.ac.jp

ABSTRACT

In mice, the number of intestinal villous columnar epithelium cells that incorporate abnormal prion protein (PrPSc) decreases significantly after weaning. In this study, the dynamics of PrPSc uptake during the growth of hamsters were investigated by inoculating scrapie 263K agent orally into suckling and weanling Syrian hamsters and estimating the number of PrPSc-positive villous epithelium cells immunohistochemically. The number of PrPSc-positive cells declined significantly as the hamsters aged. The present results suggest that a tendency toward decline of PrPSc-positive cells with increasing age might be a common phenomenon among the superfamily Muridae.

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