Enumeration of viable Enterococcus faecalis, a predominant apical periodontitis pathogen, using propidium monoazide and quantitative real-time polymerase chain reaction
Version of Record online: 27 DEC 2011
© 2011 The Societies and Blackwell Publishing Asia Pty Ltd
Microbiology and Immunology
Volume 55, Issue 12, pages 889–892, December 2011
How to Cite
Takahashi, Y., Yoshida, A., Nagayoshi, M., Kitamura, C., Nishihara, T., Awano, S. and Ansai, T. (2011), Enumeration of viable Enterococcus faecalis, a predominant apical periodontitis pathogen, using propidium monoazide and quantitative real-time polymerase chain reaction. Microbiology and Immunology, 55: 889–892. doi: 10.1111/j.1348-0421.2011.00390.x
- Issue online: 27 DEC 2011
- Version of Record online: 27 DEC 2011
- Accepted manuscript online: 17 OCT 2011 05:38AM EST
- Received 28 June 2011; revised 2 September 2011; accepted 25 September 2011.
- apical periodontitis;
- Enterococcus faecalis;
- propidium monoazide;
- viable cell
To discriminate between viable and non-viable Enterococcus faecalis, the predominant pathogen in apical periodontitis, a real-time PCR method combined with propidium monoazide (PMA) was developed and evaluated. PMA had no antimicrobial effect on E. faecalis cells and permitted enumeration of both viable and non-viable cells. Therefore, E. faecalis cells from the root canals of nine patients with apical periodontitis were analyzed to evaluate the diagnostic usefulness of this approach. Viable and non-viable E. faecalis cells were successfully discriminated in these clinical specimens. A real-time PCR assay combined with PMA will contribute to the precise diagnosis of apical periodontitis.