Anti-inflammatory properties of intestinal Bifidobacterium strains isolated from healthy infants
Article first published online: 13 FEB 2012
© 2012 The Societies and Blackwell Publishing Asia Pty Ltd
Microbiology and Immunology
Volume 56, Issue 1, pages 27–39, January 2012
How to Cite
Khokhlova, E. V., Smeianov, V. V., Efimov, B. A., Kafarskaia, L. I., Pavlova, S. I. and Shkoporov, A. N. (2012), Anti-inflammatory properties of intestinal Bifidobacterium strains isolated from healthy infants. Microbiology and Immunology, 56: 27–39. doi: 10.1111/j.1348-0421.2011.00398.x
- Issue published online: 13 FEB 2012
- Article first published online: 13 FEB 2012
- Accepted manuscript online: 1 NOV 2011 04:25AM EST
- Received 3 August 2011; revised 13 October 2011; accepted 19 October 2011.
- inflammatory bowel disease;
- anti-inflammatory activity;
- nuclear factor-κ light chain enhancer of activated B cells
Certain Bifidobacterium strains have been shown to inhibit inflammatory responses in intestinal epithelial cells. However, the precise mechanisms of these effects, including the chemical nature of the active compounds, remain to be elucidated. Here partial characterization of the anti-inflammatory properties of Bifidobacterium strains isolated from feces of healthy infants is reported. It was found that conditioned media (CM) of all strains studied are capable of attenuating tumor necrosis factor-α (TNF-α) and lipopolysaccharide- (LPS) induced inflammatory responses in the HT-29 cell line. In contrast, neither killed bifidobacterial cells, nor cell-free extracts showed such activities. Further investigations resulted in attribution of this activity to heat-stable, non-lipophilic compound(s) resistant to protease and nuclease treatments and of molecular weight less than 3 kDa. The anti-inflammatory effects were dose- and time-dependent and associated with inhibition of IκB phosphorylation and nuclear factor-κ light chain enhancer of activated B cells (NF-κB)-dependent promoter activation. The combined treatments of cells with CMs and either LPS or TNF-α, but not with CMs alone, resulted in upregulation of transforming growth factor-β1, IκBζ, and p21CIP mRNAs. Our data suggest certain species-specificities of the anti-inflammatory properties of bifidobacteria. This observation should prompt additional validation studies using larger set of strains and employing the tools of comparative genomics.