Acute respiratory infections due to enterovirus 68 in Yamagata, Japan between 2005 and 2010
Version of Record online: 13 MAR 2012
© 2012 The Societies and Blackwell Publishing Asia Pty Ltd
Microbiology and Immunology
Volume 56, Issue 2, pages 139–143, February 2012
How to Cite
Ikeda, T., Mizuta, K., Abiko, C., Aoki, Y., Itagaki, T., Katsushima, F., Katsushima, Y., Matsuzaki, Y., Fuji, N., Imamura, T., Oshitani, H., Noda, M., Kimura, H. and Ahiko, T. (2012), Acute respiratory infections due to enterovirus 68 in Yamagata, Japan between 2005 and 2010. Microbiology and Immunology, 56: 139–143. doi: 10.1111/j.1348-0421.2012.00411.x
- Issue online: 13 MAR 2012
- Version of Record online: 13 MAR 2012
- Accepted manuscript online: 7 FEB 2012 04:52AM EST
- Received 21 September 2011; revised 2 December 2011; accepted 7 December 2011.
- Acute respiratory infection;
- enterovirus 68;
- reverse transcription polymerase chain reaction;
- virus isolation
To clarify the epidemiology of enterovirus 68 (EV68), which is one of the most rarely identified enteroviruses, virus isolation and molecular screening using RT-PCR was performed on 6307 respiratory specimens collected at pediatric clinics in Yamagata, Japan between 2005 and 2010. In the years 2005–2009, 10, 1, 2, 0, and 2 (40) EV68-positive cases, respectively, were identified by RT-PCR. In 2010, 40 cases were identified altogether: 2 by isolation only, 26 by RT-PCR only, and 12 by both isolation and RT-PCR. Phylogenetic analysis indicated that plural genetically distinct clusters co-circulated. These results suggest that that difficulty in EV68 isolation leads to an underestimation of the prevalence of EV68 infections.