Genetic characterization of swine influenza viruses isolated in Japan between 2009 and 2012

Authors

  • Aya Matsuu,

    1. Research Teams for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization, 3–1-5 Kannondai, Tsukuba, Ibaraki 305–0856
    2. Thailand-Japan Zoonotic Diseases Collaboration Center, Kasertlang Chatuchak, Bangkok 10900, Thailand
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  • Yuko Uchida,

    1. Research Teams for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization, 3–1-5 Kannondai, Tsukuba, Ibaraki 305–0856
    2. Thailand-Japan Zoonotic Diseases Collaboration Center, Kasertlang Chatuchak, Bangkok 10900, Thailand
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  • Nobuhiro Takemae,

    1. Research Teams for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization, 3–1-5 Kannondai, Tsukuba, Ibaraki 305–0856
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  • Takahiro Mawatari,

    1. Yamagata Prefectural Central Livestock Health and Sanitation Office, 736 Ooaza Urushiyama, Yamagata, Yamagata 990–2161
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  • Shuji Kasai Yoneyama,

    1. Central Animal Hygiene Service Center of Tochigi Prefecture, 6–8 Hiraide kogyo-danchi, Utsunomiya, Tochigi 321–0905
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  • Tomoe Kasai,

    1. Osaka Prefectural Livestock Hygiene Service Center, 1–59 Rinkuouraikita Izumisano, Osaka 598–0048
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  • Ryoko Nakamura,

    1. Mie Chuoh Livestock Hygiene Service Center, 1742–1 Isshindenkozubeta Tsu, Mie 514–0061
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  • Mariko Eto,

    1. Animal Quarantine Service, Ministry of Agriculture, Forestry, and Fisheries, 11–1 Haramachi, Yokohama Isogo-ku, Kanagawa 235–0008, Japan
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  • Takehiko Saito

    1. Research Teams for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization, 3–1-5 Kannondai, Tsukuba, Ibaraki 305–0856
    2. Thailand-Japan Zoonotic Diseases Collaboration Center, Kasertlang Chatuchak, Bangkok 10900, Thailand
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Takehiko Saito, Research Teams for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan. Tel. and Fax: +81 29 838 7914, email: taksaito@affrc.go.jp

ABSTRACT

Eleven swine influenza viruses (SIVs) isolated from pigs in Japanese institutions between 2009 and 2012 were genetically characterized. Seven H1N1 were shown to have originated from A(H1N1)pdm09 viruses. Two H1N2 viruses contained H1 and N2 genes of Japanese H1N2 SIV origin together with internal genes of A(H1N1)pdm09 viruses. Two H3N2 viruses isolated during animal quarantine were identified as triple reassortant H3N2 viruses maintained among pigs in North America. This study shows that A(H1N1)pdm09 viruses and their reassortant strains are already present in domestic pigs in Japan and that novel SIVs are possibly being imported from abroad.

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