These authors contributed equally to this work and should be considered as co-first author.
Critical role of Toll-like receptor 2 in Bacteroides fragilis-mediated immune responses in murine peritoneal mesothelial cells
Version of Record online: 23 OCT 2012
© 2012 The Societies and Wiley Publishing Asia Pty Ltd
Microbiology and Immunology
Volume 56, Issue 11, pages 782–788, November 2012
How to Cite
Kim, T.-H., Lee, K.-B., Kang, M.-J. and Park, J.-H. (2012), Critical role of Toll-like receptor 2 in Bacteroides fragilis-mediated immune responses in murine peritoneal mesothelial cells. Microbiology and Immunology, 56: 782–788. doi: 10.1111/j.1348-0421.2012.00505.x
- Issue online: 23 OCT 2012
- Version of Record online: 23 OCT 2012
- Accepted manuscript online: 31 AUG 2012 09:16AM EST
- Received 9 April 2012; revised 6 August 2012; accepted 23 August 2012.
- Bacteroides fragilis;
- immune responses;
- peritoneal mesothelial cell;
- Toll-like receptor 2
In this study, the role of Toll-like receptor 2 (TLR2) in immune responses of murine peritoneal mesothelial cells against Bacteroides fragilis was investigated. Enzyme linked immunosorbent assay was used to measure cytokines and chemokines. Activation of nuclear factor κB (NF-κB-α) and mitogen-activated protein kinases (MAP kinases) was investigated by western blot analysis. B. fragilis induced production of interleukin-6, chemokine (C-X-C motif) ligand 1 (CXCL1) and chemokine (C-C motif) ligand 2 (CCL2) in wild type peritoneal mesothelial cells; this was impaired in TLR2-deficient cells. In addition, in response to B. fragilis, phosphorylation of inhibitory NF-κB-α and c-Jun N-terminal kinase mitogen-activated protein kinase (MAPK) was induced in wild type mesothelial cells, but not in TLR2-deficient cells,. Inhibitor assay revealed that NF-κB and MAPKs are essential for B. fragilis-induced production of CXCL1 and CCL2 in mesothelial cells. These findings suggest that TLR2 mediates immune responses in peritoneal mesothelial cells in response to B. fragilis.