Antigen 85A and mycobacterial DNA-binding protein 1 are targets of immunoglobulin G in individuals with past tuberculosis

Authors

  • Mayuko Osada-Oka,

    1. Department of Bacteriology, Osaka City University Graduate School of Medicine, 1-4-3 Abeno-ku, Osaka 545-8585
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  • Yoshitaka Tateishi,

    1. Department of Bacteriology, Osaka City University Graduate School of Medicine, 1-4-3 Abeno-ku, Osaka 545-8585
    2. Department of Respiratory Medicine, National Hospital Organization Toneyama National Hospital, 5-1-1 Toneyama, Toyonaka, Osaka 560-8552
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  • Yukio Hirayama,

    1. Department of Bacteriology, Osaka City University Graduate School of Medicine, 1-4-3 Abeno-ku, Osaka 545-8585
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  • Yuriko Ozeki,

    1. Department of Bacteriology, Osaka City University Graduate School of Medicine, 1-4-3 Abeno-ku, Osaka 545-8585
    2. Department of Food and Nutrition, Sonoda Women's University, 7-29-1 Minamitsukaguchi-cho, Amagasaki, Hyogo 661-0012
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  • Mamiko Niki,

    1. Department of Bacteriology, Osaka City University Graduate School of Medicine, 1-4-3 Abeno-ku, Osaka 545-8585
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  • Seigo Kitada,

    1. Department of Respiratory Medicine, National Hospital Organization Toneyama National Hospital, 5-1-1 Toneyama, Toyonaka, Osaka 560-8552
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  • Ryoji Maekura,

    1. Department of Respiratory Medicine, National Hospital Organization Toneyama National Hospital, 5-1-1 Toneyama, Toyonaka, Osaka 560-8552
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  • Kunio Tsujimura,

    1. Department of Infectious Diseases, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192
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  • Yukio Koide,

    1. Department of Infectious Diseases, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192
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  • Naoya Ohara,

    1. Department of Oral Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikada-cho, Kita-ku, Okayama 700-8558
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  • Taro Yamamoto,

    1. Department of International Health, Institute of Tropical Medicine, The Global Center of Excellence, Nagasaki University, Nagasaki 852-8523
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  • Kazuo Kobayashi,

    1. Department of Immunology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan
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  • Sohkichi Matsumoto

    Corresponding author
    • Department of Bacteriology, Osaka City University Graduate School of Medicine, 1-4-3 Abeno-ku, Osaka 545-8585
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Correspondence

Sohkichi Matsumoto, Department of Bacteriology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan.

Tel: +81 6 6645 3745; fax: +81 6 6645 3746; email: sohkichi@med.osaka-cu.ac.jp

Abstract

Development of accurate methods for predicting progression of tuberculosis (TB) from the latent state is recognized as vitally important in controlling TB, because a majority of cases develop from latent infections. Past TB that has never been treated has a higher risk of progressing than does latent Mycobacterium tuberculosis infection in patients who have previously received treatment. Antibody responses against 23 kinds of M. tuberculosis proteins in individuals with past TB who had not been medicated were evaluated. These individuals had significantly higher concentrations of antibodies against Antigen 85A and mycobacterial DNA-binding protein 1 (MDP1) than did those with active TB and uninfected controls. In addition, immunohistochemistry revealed colocalization of tubercle bacilli, antigen 85 and MDP1 inside tuberculous granuloma lesions in an asymptomatic subject, showing that M. tuberculosis in lesions expresses both antigen 85 and MDP1. Our study suggests the potential usefulness of measuring antibody responses to antigen 85A and MDP1 for assessing the risk of TB progression.

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