• Open Access

Reduced transthyretin expression in sera of lung cancer

Authors

  • Liyun Liu,

    1. Key laboratory for Cell Proliferation and Regulation Biology, Ministry of Education, Beijing Normal University;
    2. Universities’ Confederated Institute of Proteomics, Beijing 100875;
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  • Jifu Liu,

    1. Department of Thoracic Surgery, General Hospital of Beijing Unit, PLA, Beijing 100700, China
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  • Songwei Dai,

    1. Key laboratory for Cell Proliferation and Regulation Biology, Ministry of Education, Beijing Normal University;
    2. Universities’ Confederated Institute of Proteomics, Beijing 100875;
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  • Xiaomin Wang,

    1. Key laboratory for Cell Proliferation and Regulation Biology, Ministry of Education, Beijing Normal University;
    2. Universities’ Confederated Institute of Proteomics, Beijing 100875;
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  • Shanshan Wu,

    1. Department of Thoracic Surgery, General Hospital of Beijing Unit, PLA, Beijing 100700, China
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  • Jianing Wang,

    1. Key laboratory for Cell Proliferation and Regulation Biology, Ministry of Education, Beijing Normal University;
    2. Universities’ Confederated Institute of Proteomics, Beijing 100875;
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  • Lingyun Huang,

    1. Key laboratory for Cell Proliferation and Regulation Biology, Ministry of Education, Beijing Normal University;
    2. Universities’ Confederated Institute of Proteomics, Beijing 100875;
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  • Xueyuan Xiao,

    Corresponding author
    1. Key laboratory for Cell Proliferation and Regulation Biology, Ministry of Education, Beijing Normal University;
    2. Universities’ Confederated Institute of Proteomics, Beijing 100875;
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  • Dacheng He

    1. Key laboratory for Cell Proliferation and Regulation Biology, Ministry of Education, Beijing Normal University;
    2. Universities’ Confederated Institute of Proteomics, Beijing 100875;
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To whom correspondence should be addressed. E-mail: xyxiao@bnu.edu.cn, dhe@bnu.edu.cn

Abstract

Lung cancer is a leading cause of cancer death worldwide, and very few specific biomarkers can be used in its clinical diagnosis. Using surface-enhanced laser desorption–ionization time-of-flight mass spectrometry (MS) to find novel serum biomarkers for lung cancer, we analyzed 227 serum samples, including 146 lung cancers, 41 benign lung diseases and 40 normal individuals. Three peaks, at 13.78, 13.90 and 14.07 k m/z, were significantly lower in lung cancer sera compared with sera from normal individuals (P < 0.001), whereas these peaks were higher than those in the sera of benign lung diseases (P < 0.001). The peaks were identified as native transthyretin (TTR) and its two variants by one-dimensional polyacrylamide gel electrophoresis, ESI-MS/MS, immunoprecipitation and western blot analysis. An enzyme-linked immunosorbent assay indicated that TTR levels were consistent with surface-enhanced laser desorption–ionization analysis in all groups tested. It gave 78.5% sensitivity and 77.5% specificity for lung cancer versus normal at the cut-off point 115 µg/mL, and 66.7% sensitivity and 64.4% specificity for lung cancer versus benign lung diseases at the cut-off point 88.5 µg/mL. Therefore, TTR may be useful as a biomarker to improve the diagnosis of lung cancer. (Cancer Sci 2007; 98: 1617–1624)

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