SEARCH

SEARCH BY CITATION

Fig. S1. (A) HepG2, HepG2-3X and HepG2-4X cells were cultured upon treatment with doxorubicin (1 µg/ml) for 24 h. Expression levels of the p53 protein were determined by Western blotting using anti-p53 antibody. a-tubulin levels were used as loading control in each lane. (B) HepG2 and HepG2-4X cells were cultivated on a cover slip. Cells were fixed with cold methanol-acetone (1:1) and examined for subcellular HBx and p53 distribution under confocal microscope. The scale bar indicates 10 µg. (C) HepG2 and HepG2-4X cells were treated with 1 µg/ml of doxorubicin for 24 h. Subcellular localizations of HBx and p53 protein were visualized by immunofluorescence staining using anti-HBx antibody (green) and p53 (red) under confocal microscope. The scale bar indicate 10 µg.

Please note: Blackwell Publishing are not responsible for the content or functionality of any supplementary materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.

FilenameFormatSizeDescription
CAS_754_sm_FigS2.ppt1623KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.