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Fig. S1. The lower proliferation of 15-LO-1 expressing cells can be reversed with the use of a specific inhibitor PD146176. HCT-116 cells stably expressing 15-LO-1 and empty vector expressing control cells were plated and allowed to grow for 48 h. The cells were then either treated or not with 1 &mgr;m PD146176 for 48 h. The cellular proliferation was then determined by an MTT assay and read colorimetrically at 570 nm.

Fig. S2. 15-LO-1 expression reduces migration across a Transwell membrane in HT-29 cells. A Transwell migration assay was done in the presence of serum as a chemoattractant. 15-LO-1 transfected HT-29 cells migrated through the 8 m pores of the Transwell in significantly less numbers (**P < 0.01) compared to the control cells.  The bar diagram represents the number of cells that could migrate through the Transwell in the experimental and control cells lines. Error bars represent SD from two independent experiments carried out in four replicates.

Fig. S3. 15-LO-1 expression reduces invasion through Matrigel in HT-29 cells. A Transwell invasion assay assaying the ability of the HT-29 cells to invade through Matrigel, a reconstituted basement membrane was carried out. 15-LO-1 expression significantly reduces (**< 0.01) the ability of HT-29 cells to invade through the basement membrane in vitro compared to empty vector expressing control cells. The bar diagram represents the number of cells that could invade through the Matrigel coated Transwell inserts in the experimental and control cells lines. Error bars represent SD from two independent experiments carried out in four replicates.

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