Tumor–stromal interactions with direct cell contacts enhance proliferation of human pancreatic carcinoma cells
Article first published online: 19 AUG 2009
© 2009 Japanese Cancer Association
Volume 100, Issue 12, pages 2309–2317, December 2009
How to Cite
Fujita, H., Ohuchida, K., Mizumoto, K., Egami, T., Miyoshi, K., Moriyama, T., Cui, L., Yu, J., Zhao, M., Manabe, T. and Tanaka, M. (2009), Tumor–stromal interactions with direct cell contacts enhance proliferation of human pancreatic carcinoma cells. Cancer Science, 100: 2309–2317. doi: 10.1111/j.1349-7006.2009.01317.x
- Issue published online: 11 NOV 2009
- Article first published online: 19 AUG 2009
- (Received February 24, 2009/Revised June 27, 2009; August 06, 2009/Accepted August 12, 2009/Online publication September 7, 2009)
Fig. S1. (a) Propidium iodide (PI) fluorescence intensity of green fluorescent protein (GFP)-SUIT-2 cells. (b) The GFP fluorescence intensity was correlated with the PI fluorescence intensity (Spearman’s rank-correlation coefficient: 0.998, P < 0.0001). (c) GFP-SUIT-2 cells expressed similar levels of fluorescence intensity to monocultured cells, regardless of the number of co-cultured pancreatic stellate cells (PSCs). (c,d) Regression analysis confirmed that the fluorescence intensity of GFP-SUIT-2 cells was correlated with the number of cells, despite the coexistence of myofibroblasts and their morphological alterations (Spearman’s rank-correlation coefficient: 0.993, P < 0.0001).
Fig. S2. (a) Representative microphotographs of green fluorescent protein (GFP)-SUIT-2 cells in monoculture (left), indirect co-culture with MRC5 fibroblasts (center), and direct co-culture with MRC5 fibroblasts (right). Monocultured GFP-SUIT-2 cells were almost round in shape, whereas co-cultured cells exhibit a fibroblastoid morphology. (b) GFP-expressing cancer cells and GFP-negative MRC5 fibroblasts were isolated using a cell sorter. (c) The Notch-1 levels were only elevated in directly co-cultured MRC5 fibroblasts. (d,e) The hairy and enhancer-of-split homolog-1 (Hes-1) and Jagged-1 mRNA levels in directly co-cultured cells were dramatically elevated compared with those in indirectly co-cultured and monocultured cells.
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