Present address: Laboratory of Immunobiology, Department of Pharmacy, School of Pharmacy, Hyogo University of Health Sciences, Kobe, Japan.
LIM domain-containing adaptor, leupaxin, localizes in focal adhesion and suppresses the integrin-induced tyrosine phosphorylation of paxillin
Article first published online: 12 OCT 2009
© 2009 Japanese Cancer Association
Volume 101, Issue 2, pages 363–368, February 2010
Total views since August 2010: 92
How to Cite
Tanaka, T., Moriwaki, K., Murata, S. and Miyasaka, M. (2010), LIM domain-containing adaptor, leupaxin, localizes in focal adhesion and suppresses the integrin-induced tyrosine phosphorylation of paxillin. Cancer Science, 101: 363–368. doi: 10.1111/j.1349-7006.2009.01398.x
- Issue published online: 29 JAN 2010
- Article first published online: 12 OCT 2009
- (Received August 7, 2009/Revised September 30, 2009, 2008/Accepted October 2, 2009/Online publication November 16, 2009)
- 1Cell adhesion: the molecular basis of tissue architecture and morphogenesis. Cell 1996; 84: 345–57..
- 2Integrins: bidirectional, allosteric signaling machines. Cell 2002; 110: 673–87..
- 3Genetic and cell biological analysis of integrin outside-in signaling. Genes Dev 2009; 23: 397–418., , .
- 4Integrins and signal transduction pathways: the road taken. Science 1995; 268: 233–9., .
- 5Environmental sensing through focal adhesions. Nat Rev Mol Cell Biol 2009; 10: 21–33., , .
- 6Paxillin and focal adhesion signalling. Nat Cell Biol 2000; 2: E231–6..
- 7Paxillin: adapting to change. Physiol Rev 2004; 84: 1315–39., .
- 8Paxillin: a focal adhesion-associated adaptor protein. Oncogene 2001; 20: 6459–72..
- 9Leupaxin is a novel LIM domain protein that forms a complex with PYK2. J Biol Chem 1998; 273: 11709–13., , .
- 10The LIM protein leupaxin is enriched in smooth muscle and functions as an serum response factor cofactor to induce smooth muscle cell gene transcription. Circ Res 2008; 102: 1502–11., , , , .
- 11Interaction of Pyk2 and PTP-PEST with leupaxin in prostate cancer cells. Am J Physiol Cell Physiol 2007; 292: C2288–96., , , .
- 12Leupaxin, a novel coactivator of the androgen receptor, is expressed in prostate cancer and plays a role in adhesion and invasion of prostate carcinoma cells. Mol Endocrinol 2008; 22: 1606–21., , et al.
- 13Leupaxin is a critical adaptor protein in the adhesion zone of the osteoclast. J Bone Miner Res 2003; 18: 669–85., , et al.
- 14Leupaxin negatively regulates B cell receptor signaling. J Biol Chem 2007; 282: 27181–9., .
- 15BodyMap: a human and mouse gene expression database. Nucleic Acids Res 2000; 28: 136–8., , , .
- 16Expression profile of active genes in mouse lymph node high endothelial cells. Int Immunol 1999; 11: 1989–98., , et al.
- 17Gene expression profiling of mucosal addressin cell adhesion molecule-1 + high endothelial venule cells (HEV) and identification of a leucine-rich HEV glycoprotein as a HEV marker. J Immunol 2002; 168: 1050–9., , et al.
- 18pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res 1990; 18: 5322., .
- 19Development of a cell-free binding assay for rat ICAM-1/LFA-1 interactions using a novel anti-rat LFA-1 monoclonal antibody and comparison with a cell-based assay. J Immunol Methods 1999; 228: 69–79., , .
- 20Inhibition of cell spreading by expression of the C-terminal domain of focal adhesion kinase (FAK) is rescued by coexpression of Src or catalytically inactive FAK: a role for paxillin tyrosine phosphorylation. Mol Cell Biol 1997; 17: 6906–14., , , .
- 21Interaction of Hic-5, A senescence-related protein, with focal adhesion kinase. J Biol Chem 1998; 273: 26516–21., , , , , .
- 22Hic-5-reduced cell spreading on fibronectin: competitive effects between paxillin and Hic-5 through interaction with focal adhesion kinase. Mol Cell Biol 2001; 21: 5332–45., , , , .
- 23Identification of LIM3 as the principal determinant of paxillin focal adhesion localization and characterization of a novel motif on paxillin directing vinculin and focal adhesion kinase binding. J Cell Biol 1996; 135: 1109–23., , .
- 24Characterization of a focal adhesion protein, Hic-5, that shares extensive homology with paxillin. J Cell Sci 1999; 112: 181–90., , .
- 25The paxillin LD motifs. FEBS Lett 2002; 513: 114–8., , .
- 26Serine and threonine phosphorylation of the paxillin LIM domains regulates paxillin focal adhesion localization and cell adhesion to fibronectin. Mol Biol Cell 1998; 9: 1803–16., , .
- 27Molecular cloning of human Hic-5, a potential regulator involved in signal transduction and cellular senescence. Mol Carcinog 2000; 27: 177–83., , , , .
- 28Integrin cytoplasmic domains mediate inside-out signal transduction. J Cell Biol 1994; 124: 1047–59., , et al.
- 29The role of focal adhesion kinase binding in the regulation of tyrosine phosphorylation of paxillin. J Biol Chem 1999; 274: 36684–92., , et al.
- 30pp125FAK-dependent tyrosine phosphorylation of paxillin creates a high-affinity binding site for Crk. Mol Cell Biol 1995; 15: 2635–45., .
- 31Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL. J Biol Chem 1995; 270: 5039–47., , et al.
- 32Specific and redundant roles of Src and Fyn in organizing the cytoskeleton. Nature 1995; 376: 267–71., , .