In vivo bioimaging tracks conditionally replicative adenoviral replication and provides an early indication of viral antitumor efficacy
Article first published online: 20 OCT 2009
© 2009 Japanese Cancer Association
Volume 101, Issue 2, pages 474–481, February 2010
How to Cite
Davydova, J., Gavrikova, T., Brown, E. J., Luo, X., Curiel, D. T., Vickers, S. M. and Yamamoto, M. (2010), In vivo bioimaging tracks conditionally replicative adenoviral replication and provides an early indication of viral antitumor efficacy. Cancer Science, 101: 474–481. doi: 10.1111/j.1349-7006.2009.01407.x
- Issue published online: 29 JAN 2010
- Article first published online: 20 OCT 2009
- (Received September 2, 2009/Revised October 8, 2009/Accepted October 12, 2009/Online publication November 8, 2009)
Fig. S1. Cox2CRAdΔE3ADPLuc showed luminescence-based imaging in spontaneously developed distant metastases in a nude mouse. (a) By day 13 after intratumoral treatment with Cox2CRAdΔE3ADPLuc (4 × 109 vp/tumor), one mouse bearing an A549 subcutaneous xenograft developed ascites, and bioluminescent signal was detected from the abdominal area. (b) On day 20 after adenovirus (Ad) treatment, the mouse was sacrificed after acquiring the whole-body luciferase imaging, and the peritoneal cavity was opened. (c) To confirm the origin of the bioluminescent signal in the peritoneal cavity, the organs were isolated and placed on a petri dish and re-imaged ex vivo. The source of bioluminescent signal was from distant tumor metastases in the intestines, stomach, diaphragm, and peritoneum.
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