Fig. S1. Expression of PRNCR1 in normal prostate epithelial cells (PrEC) and prostate cancer cell line LNCaP. The expression of PRNCR1 in PrEC cells was weak. Primer set A: 5′-ACAAAATTTGCATTTGTTGGA-3′ and 5′-GTAAATCCCCAGAGCAGAGTC-3′, and Primer set B: 5′-ATTGTTCCCAGTTCTCTGGAC-3′ and 5′-GGAGGAAGTGGAGTAGGTATAAGAG-3′. ACTB expression served to normalize the input cDNA.

Fig. S2. The cell viability of PRNCR1 attenuated prostate cancer cells. The MTT assay was performed at 72&00A0;h after transfection with siRNA-duplex into LNCaP (left) or PC-3 (right) cells. Y-axis: absorbance (ABS) at 490 nm and at 630 nm as a reference. These experiments were carried out in triplicate (P-value; Student’s t-test). Error bars represent the mean ± SD.

Table S1. Detailed genotyping results of the candidate region from rs1902431 (128,156,258 bp) to rs7825340 (128,178,311 bp).

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