Association of a novel long non-coding RNA in 8q24 with prostate cancer susceptibility
Version of Record online: 28 SEP 2010
© 2010 Japanese Cancer Association
Volume 102, Issue 1, pages 245–252, January 2011
How to Cite
Chung, S., Nakagawa, H., Uemura, M., Piao, L., Ashikawa, K., Hosono, N., Takata, R., Akamatsu, S., Kawaguchi, T., Morizono, T., Tsunoda, T., Daigo, Y., Matsuda, K., Kamatani, N., Nakamura, Y. and Kubo, M. (2011), Association of a novel long non-coding RNA in 8q24 with prostate cancer susceptibility. Cancer Science, 102: 245–252. doi: 10.1111/j.1349-7006.2010.01737.x
- Issue online: 15 DEC 2010
- Version of Record online: 28 SEP 2010
- Accepted manuscript online: 3 SEP 2010 12:32AM EST
- (Received June 29, 2010/Revised August 25, 2010/Accepted August 30, 2010/Accepted manuscript online September 3, 2010/Article first published online September 28, 2010)
Fig. S1. Expression of PRNCR1 in normal prostate epithelial cells (PrEC) and prostate cancer cell line LNCaP. The expression of PRNCR1 in PrEC cells was weak. Primer set A: 5′-ACAAAATTTGCATTTGTTGGA-3′ and 5′-GTAAATCCCCAGAGCAGAGTC-3′, and Primer set B: 5′-ATTGTTCCCAGTTCTCTGGAC-3′ and 5′-GGAGGAAGTGGAGTAGGTATAAGAG-3′. ACTB expression served to normalize the input cDNA.
Fig. S2. The cell viability of PRNCR1 attenuated prostate cancer cells. The MTT assay was performed at 72&00A0;h after transfection with siRNA-duplex into LNCaP (left) or PC-3 (right) cells. Y-axis: absorbance (ABS) at 490 nm and at 630 nm as a reference. These experiments were carried out in triplicate (P-value; Student’s t-test). Error bars represent the mean ± SD.
Table S1. Detailed genotyping results of the candidate region from rs1902431 (128,156,258 bp) to rs7825340 (128,178,311 bp).
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