Present address: Section of Animal Research, Center of Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, Japan.
Involvement of retrotransposition of long interspersed nucleotide element-1 in skin tumorigenesis induced by 7,12-dimethylbenz[a]anthracene and 12-O-tetradecanoylphorbol-13-acetate
Article first published online: 19 SEP 2011
© 2011 Japanese Cancer Association
Volume 102, Issue 11, pages 2000–2006, November 2011
How to Cite
Okudaira, N., Goto, M., Yanobu-Takanashi, R., Tamura, M., An, A., Abe, Y., Kano, S., Hagiwara, S., Ishizaka, Y. and Okamura, T. (2011), Involvement of retrotransposition of long interspersed nucleotide element-1 in skin tumorigenesis induced by 7,12-dimethylbenz[a]anthracene and 12-O-tetradecanoylphorbol-13-acetate. Cancer Science, 102: 2000–2006. doi: 10.1111/j.1349-7006.2011.02060.x
- Issue published online: 23 OCT 2011
- Article first published online: 19 SEP 2011
- Accepted manuscript online: 9 AUG 2011 12:41PM EST
- (Received April 21, 2011/Revised June 10, 2011; July 19, 2011/Accepted August 1, 2011/Accepted manuscript online August 9, 2011/Article first published online September 19, 2011)
Fig. S1. No cytotoxicity was detected in cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) and 7,12-dimethylbenz[a]anthracene (DMBA).
Fig. S2. Selection of human long interspersed nucleotide element-1–enhanced green fluorescent protein (hL1-EGFP) mouse strains suitable for carcinogenesis experiments.
Fig. S3. Southern blot analysis of copy numbers of transgenes in #4 and #67 in mice carrying human long interspersed nucleotide element-1 and enhanced green fluorescent protein (hL1-EGFP).
Fig. S4. Long interspersed nucleotide element-1 retrotransposition (L1-RTP) in nevi. Eight nevi samples were analyzed by PCR-based assay.
Fig. S5. Forced expression of constitutively activated form of Stat3 did not induce long interspersed nucleotide element-1 retrotransposition (L1-RTP).
Fig. S6. Forced expression of activated H-ras did not phosphorylate Stat3.
Fig. S7. Aryl hydrocarbon receptor (AhR) is required for long interspersed nucleotide element-1 retrotransposition (L1-RTP) by genotoxic carcinogens.
Fig. S8. No apparent abnormality of Gsta4 gene in tumors induced by 7,12-dimethylbenz[a]anthracene/12-O-tetradecanoylphorbol-13-acetate (DMBA/TPA).
Table S1. Nucleotide sequences of siRNAs targeting aryl hydrocarbon receptor (AhR) and AhR nuclear translocator 1 (ARNT1).
Table S2. Quantitative PCR of copy numbers of transgene in #4 and #67 in mice carrying human long interspersed nucleotide element-1 and enhanced green fluorescent protein (L1-EGFP).
Data S1. Full details of experimental methods used in this work.
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