Semi-quantitative evaluation of CD44+/CD24− tumor cell distribution in breast cancer tissue using a newly developed fluorescence immunohistochemical staining method
Article first published online: 14 SEP 2011
© 2011 Japanese Cancer Association
Volume 102, Issue 12, pages 2132–2138, December 2011
How to Cite
Kai, M., Onishi, H., Souzaki, M., Tanaka, H., Kubo, M., Tanaka, M. and Katano, M. (2011), Semi-quantitative evaluation of CD44+/CD24− tumor cell distribution in breast cancer tissue using a newly developed fluorescence immunohistochemical staining method. Cancer Science, 102: 2132–2138. doi: 10.1111/j.1349-7006.2011.02063.x
- Issue published online: 20 NOV 2011
- Article first published online: 14 SEP 2011
- Accepted manuscript online: 12 AUG 2011 01:02PM EST
- (Received April 5, 2011/Revised July 28, 2011/Accepted August 8, 2011/Accepted manuscript online August 12, 2011)
Fig. S1. The CD44+/CD24− and CD44+/CD24+ cells from MCF-7 were collected by the cell sorter separately.
Fig. S2. The Shh and Gli1 protein expressions are co-located with the CD44+/CD24− cells in breast cancer tissue (original magnification, ×630).
Table S1. Twenty thousand CD44+/CD24− cells could make tumors, while 100 000 control whole cells could make tumors.
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