Transforming growth factor-β-stimulated clone-22 is a negative-feedback regulator of Ras / Raf signaling: Implications for tumorigenesis
Article first published online: 1 NOV 2011
© 2011 Japanese Cancer Association
Volume 103, Issue 1, pages 26–33, January 2012
How to Cite
Nakamura, M., Kitaura, J., Enomoto, Y., Lu, Y., Nishimura, K., Isobe, M., Ozaki, K., Komeno, Y., Nakahara, F., Oki, T., Kume, H., Homma, Y. and Kitamura, T. (2012), Transforming growth factor-β-stimulated clone-22 is a negative-feedback regulator of Ras / Raf signaling: Implications for tumorigenesis. Cancer Science, 103: 26–33. doi: 10.1111/j.1349-7006.2011.02108.x
- Issue published online: 6 JAN 2012
- Article first published online: 1 NOV 2011
- Accepted manuscript online: 22 SEP 2011 08:48AM EST
- (Received June 22, 2011/Revised September 5, 2011/Accepted September 19, 2011/Accepted manuscript online September 22, 2011/Article first published online November 1, 2011)
Fig. S1. Ras/Raf activation upregulated TSC-22 at both transcript and protein levels.
Fig. S2. Relative expression levels of GILZ of Ba/F3 cells (A) or NIH3T3 cells (B) transduced with H-Ras-G12V or mock were estimated by RT-PCR.
Fig. S3. Transduction with TSC-22, but not TSC22D1-1, inhibited the cell growth in NIH3T3 cells.
Fig. S4. Protein expression of TSC-22 in NIH3T3 cells transfected with small interference RNA (siRNA) for TSC-22.
Fig. S5. Effect of siRNA treatment with TSC-22 oligonucleotides on the growth and anchorage-independent colony formation of H-Ras-G12V-transduced NIH3T3 cells.
Fig. S6. Activation of Raf-1, but not STAT5, induced the nuclear translocation of TSC-22 in 293T cells.
Fig. S7. TSC-22 failed to interact physically with oncogenic H-Ras.
Fig. S8. Genotyping of WT and TSC22D1-deficient mice.
Data S1. Material and methods.
|CAS_2108_sm_dataS1.doc||56K||Supporting info item|
|CAS_2108_sm_fS2.tif||130K||Supporting info item|
|CAS_2108_sm_fS3.tif||119K||Supporting info item|
|CAS_2108_sm_fS4.tif||183K||Supporting info item|
|CAS_2108_sm_fS5.tif||152K||Supporting info item|
|CAS_2108_sm_fS6.tif||692K||Supporting info item|
|CAS_2108_sm_fS7.tif||305K||Supporting info item|
|CAS_2108_sm_fS8.tif||268K||Supporting info item|
|CAS_2108_sm_fS1.tif||214K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.