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Fig. S1. Ras/Raf activation upregulated TSC-22 at both transcript and protein levels.

Fig. S2. Relative expression levels of GILZ of Ba/F3 cells (A) or NIH3T3 cells (B) transduced with H-Ras-G12V or mock were estimated by RT-PCR.

Fig. S3. Transduction with TSC-22, but not TSC22D1-1, inhibited the cell growth in NIH3T3 cells.

Fig. S4. Protein expression of TSC-22 in NIH3T3 cells transfected with small interference RNA (siRNA) for TSC-22.

Fig. S5. Effect of siRNA treatment with TSC-22 oligonucleotides on the growth and anchorage-independent colony formation of H-Ras-G12V-transduced NIH3T3 cells.

Fig. S6. Activation of Raf-1, but not STAT5, induced the nuclear translocation of TSC-22 in 293T cells.

Fig. S7. TSC-22 failed to interact physically with oncogenic H-Ras.

Fig. S8. Genotyping of WT and TSC22D1-deficient mice.

Data S1. Material and methods.

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