• Open Access

Behavior of seeds and soil in the mechanism of metastasis: A deeper understanding

Authors


To whom correspondence should be addressed.

E-mail: lucy.mathot@igp.uu.se

Abstract

The so-called “seed and soil” hypothesis proposed by Stephen Paget in 1889 to explain the metastatic behavior of cancer cells and the homing of certain cancers to “selected” sites has been a well-recognized phenomenon for over a century. What advances have been made to increase our understanding of this phenomenon and what does it really implicate in terms of targets for therapy? (Cancer Sci 2012; 103: 626–631)

The seeds of a plant are carried in all directions; but they can only live and grow if they fall on congenial soil – Stephen Paget, 1889.[1]

Metastatic cancer arises when cancerous cells from a tumor spread from the primary lesion and form new tumors at another site in the body. Cancers that metastasize are generally more difficult to treat than those that remain at the site of origin, and have a worse prognosis. Most deaths from cancer are a result of metastases that are resistant to conventional therapies, even therapies that might have been successful in the treatment of the primary cancer.[2] This might be explained by the metastatic process itself, and the changes a tumor cell must undergo to become a successful initiator of metastases.

The metastatic process consists of several steps, as outlined in Figure 1. Initially, cells of the primary tumor, the so called “seeds” require angiogenesis for increased blood supply and the provision of growth factors. However, this is also necessary for metastasis, as tumor vascularization increases the chances for tumor cells to enter the circulation and become metastatic. For angiogenesis to be involved in metastatic cancer requires an increased vascularization of the primary tumor followed by a breakdown of the extracellular matrix (ECM) to allow tumor cells to intravasate.[3]

Figure 1.

Steps in the metastatic process. (a) primary tumor, (b) angiogenesis in primary tumor, (c) intravasation, (d) migration and extravasation, (e) colonization of new organ and (f) angiogenesis and growth of new tumor.

Following this, the circulating tumor cells must be able to form attachments to their environment; that is, to other cells and the ECM. In this way, they might eventually succeed in extravasation by proteolysis of the ECM and migration into the surrounding tissue. The tumor cell must eventually colonize the site (soil) for metastasis to be successful.[3-5] All of these processes must be completed, and done so successfully, before a metastasis is established.

Of course, these processes are regulated by many pathways, both exogenous and endogenous, and may require that the original cell from the primary tumor undergoes many changes. Interestingly, cells with different metastatic properties have been successfully isolated from the same primary tumor, indicating that the ability of the “seed” of a given tumor to metastasize is complicated even further.[6]

It is also of note that we are still unaware if the seed that can successfully metastasize is a result of an accumulation of mutations conferring an advantage on the cell and resulting in an ability to thrive elsewhere in the body, or if these metastatic seed cells are an integral part of each primary tumor from the beginning. Perhaps it is even the case that both of these hypotheses are true.

In this way, treatment of the primary tumor and the metastatic tumor are very different due to the possibility that this selection process is occurring. It might be less a matter of a seed simply being “carried in all directions,” but, rather, a complex relationship between the internal changes within the original tumor cell and the influence of the ever changing composition of the soil as it makes its journey.

What is it that decides which organs shall suffer in a case of disseminated cancer?

The importance of the “soil” in metastasis is not to be underestimated in a successful metastatic process. This “soil” may be considered as a host factor, stroma, niche or organ microenvironment.[7] It is no coincidence that certain tumors home to characteristic organs. As Stephen Paget emphasized in 1889; “the distribution of the secondary growths is not a matter of chance.”

One cannot dispute the importance of mechanical lodgment of circulating tumor cells and the resulting growth of the arrested cell, in particular when considering metastatic growth in the lung for example.[8, 9] This, however, does not explain the many clinical metastases that cannot be accounted for by circulatory patterns and mechanical lodgment of blood borne tumor cells.[10]

In fact, although the process of metastasis is highly inefficient (and only a few cells in a primary tumor are believed to be capable of forming metastases), the entry of tumor cells to the bloodstream is relatively common in cancer.[11, 12] However, it has been documented that <0.01% of circulating tumor cells eventually succeed in forming secondary growths.[11] The findings of Tarin et al.[13] that the development of secondary cancer was rare even upon direct deposition of millions of tumor cells into the vena cava (to reduce ascites from ovarian cancer) is a good indication of the importance of other factors involved.

The main patterns of seemingly “random” distant metastases are outlined in Table 1. This table does not include regional metastases because they might be explained almost exclusively by mechanical and anatomical processes. These kinds of regional metastases support the hypothesis put forward by Ewing in opposition to Paget's “seed and soil” theory that “the mechanisms of the circulation will doubtless explain most of these peculiarities.”[14]

Table 1. Typical sites of metastasis for some common solid tumors
Cancer typePrimary sitePrincipal site of metastasis
Breast adenocarcinomaBreastBone, lung, liver and brain
Small cell carcinomaLungBrain, liver and bone
Malignant melanomaSkinLung, brain and liver
Prostatic adenocarcinomaProstateBone
Testicular carcinomaTestisLiver
Colorectal adenocarcinomaColon/rectumLiver and lung
NeuroblastomaMediastinum, abdomenLiver

It is of note that Ewing could not, however, explain the strange phenomenon that the spleen escaped from this occurrence with “peculiar frequency,”[14] despite its rich blood supply. Studies have shown that the most difficult point in the metastatic process is, in fact, the initiation of growth in the secondary tissue or organ. The tumor cells that have succeeded in travelling to the site of secondary growth might fail to thrive there due to an inability to trigger the angiogenesis necessary for growth, or simply might exit the cell cycle and remain dormant.[15, 16]

Furthermore, although some cancers share the same principle metastatic sites, the time period between the tumor dissemination, organ infiltration and eventual colonization might differ greatly. This may be described as metastatic latency and is illustrated by such metastases as those from primary breast and lung malignancies. Adenocarcinomas from both breast and lung are known to metastasize to bone, liver and brain. However, while breast metastases may only be detected following years of remission, lung metastases may appear several months following initial detection and diagnosis, even while the primary tumor is a symptomless lesion.[17, 18]

This raises even more questions about the “seed and soil” relationship of tumor cell and host organ. If it is the case that an accumulation of perfect mutations for colonizing a distant organ is necessary for metastasis, perhaps this can explain the inefficiency of the process. However, is this accumulation less difficult to achieve for colonizing certain organs that regularly host metastatic tumors? What triggers the development of colonizing ability in these tumor cells? What does the “soil” need to provide to allow them to remain in a dormant state for (in some cases) many years? Finally, and, perhaps most importantly, how can we develop strategies to target these mechanisms?

General Features of Metastasis

It is suggested by Nowell that selection pressure results in the acquirement of genetic variability within developing tumors.[19] These variations confer increased malignant capabilities on the tumor cell and might result in an increased ability of some tumor cells to metastasize, compared to other non-metastatic cells within the same tumor. These selected cells might not only have an increased metastatic ability, but might also be more resistant to eradication strategies, such as chemotherapy, and normal homeostatic controls, such as anti-growth signaling and immune attack by the host.[20]

Various mechanisms, such as cellular motility and basement membrane degradation, have been implicated in the metastatic process, for example in the entry of cancer cells to the circulation. The aberrant expression of cytoskeletal modifiers, such as RhoC (which has a role in the control of cytoskeletal organization in response to extracellular factors) and Endo180 (a collagen internalization receptor implicated in invasion), can enhance dissemination in metastatic processes in a specific way.[21, 22]

In addition, the process of epithelial to mesenchymal transition (EMT), where cells lose cell–cell adhesion and dissemination of tumor cells from epithelial cells occurs, has been recognized as an important initiator of metastasis, playing a major role in invasion and intra/extravasation. Several proteins have been identified in this process. For example, Twist, a transcription factor in embryonic development, is identified by Yang et al. as an essential component of EMT induction in metastasis by repression of E-cadherin and is an attractive protein to target in metastasis.[23, 24] Similarly, the process of mesenchymal to epithelial transition, and the mechanisms involved therein, is an equally attractive target.

Aspects of the host vasculature itself are implicated in general metastatic mechanisms: for example, loss of interaction between the Duffy antigen chemokine receptor and CD82 (a known suppressor of metastasis), as well as evasion of immune surveillance through platelet binding.[5] This is perhaps also important in the avoidance of shear stress in the vasculature.[25]

The genetic background of the individual has also been a topic of discussion with regard to susceptibility of that person to metastasis. Hunter suggests that it is not only a pro-metastatic signature in tumor cells that is important, but also the polymorphisms present in the healthy cells of the individual. These genetic variations increase or decrease the likelihood that a tumor will metastasize.[26, 27] Studying the relevant polymorphisms might be a strategy for predicting the likelihood of non-specific metastasis in an individual.

The means by which the tumor cells and host tissues contribute to metastatic development are outlined in Table 2.

Table 2. Metastatic mechanisms by tumor cells and host tissue
Nature of cellMechanism promoting metastasis
Tumor cellStimulation of growth and angiogenesis
Movement and invasion
Expression of cell surface receptors for adhesion molecules
Host cellVascularization and production of growth factors
Expression of chemotactic proteins
Immune response and platelet binding

Organ Specific Metastasis

Seed: breast carcinoma cell; soil: bone.

Breast and prostate cancer are the most common carcinomas to develop bone metastases. There are characteristics of the bone niche that help to explain this tendency. Biochemical (the presence of cytokines and growth factors) and physical (acidic pH, high extracellular calcium concentration) properties of bone mean that it is a soil for tumor seeds providing a most welcoming microenvironment where they may thrive.[28]

Looking at breast carcinomas specifically, it has been shown in a number of microarray analyses that there are a small number of genes that differ consistently between the parent tumor and the metastasis. For example, in a study of primary tumor and metastases in 100 breast cancer patients, Gancberg et al.[29] found that 6% of metastases had an overexpression of Her2/neu when compared to the primary tumor. In addition, van de Vijver et al.[30] used DNA microarray analysis on breast cancer patients to develop a gene expression signature that can predict poor outcome and metastasis. The genes involved in this prognostic signature are involved in regulating cell cycle, invasion, metastasis and angiogenesis.

Kang et al.[31] identify that CXCR4 expression in breast cancer cells is a key gene in the expression signature of bone metastasizing tumor cells. Interestingly, CXCR4 is a receptor for CXCL12, a chemotactic protein preferentially expressed by stromal cells in target organs of breast metastases; that is, bone, brain, liver, lymph node and lung.[32] CXCR4 also enhances adhesion of cells expressing the protein to endothelial cells through activation of integrin, which further supports the hypothesis that the expression of CXCR4 is essential for metastatic mechanisms.[33] Therefore, DPPIV-dependent depletion of CXCL12 is a potential strategy for development of adjuvant therapy targeting metastatic breast cancer.[34]

In fact, preclinical studies have shown evidence that blockage of the CXCL12 pathway in breast cancer can be efficacious in a preventative sense; that is, it might delay the metastatic process if the patient is treated near to the time of initial primary tumor establishment.[35] However, as this strategy is not practically feasible in a clinical setting, it might not be sufficient as a targeting strategy in breast tumor metastasis.[36] However, there is both preclinical and clinical data available to suggest that the use of CXCL12 inhibition in combination with conventional therapies, such as radiotherapy and anti-angiogenic therapy, might be useful as in many cases the levels of CXCL12 increases following therapy.[36] Increased levels also correlate with increased levels of metastatic disease in rectal cancer.[37]

Therefore, this CXCR4/CXCL12 interaction relationship between both the CXCR4 expressing seed (as in metastatic breast cancer cells) and the soil (stromal cells in bone) remains an intriguing choice of target in metastasis.

Bone remodeling in metastatic breast cancer is also a mechanism to potentially target. Tumor cells release such osteoblastic growth stimulating factors as parathyroid hormone (PTHrP), which results in osteoclastic bone resorption through RANK signaling. This ultimately leads to release of growth factors, including TGFβ, for example, resulting in a pathogenic positive feedback loop with TGFβ stimulating a further increase in the expression of PTHrP from tumor “seed” cells.[38] This mechanism might even further promote metastatic development due to the pro-angiogenic and immunosuppressive properties of TGFβ.[39] Although this dual role of TGFβ has been a topic of much debate in recent years, it now appears that although it has a tumor suppressive role early in breast cancer development, overexpression at a later stage might result in anti-cancer drug resistance, as appears to be the case in patients treated with tamoxifen.[40, 41]

Indeed, there have been numerous strategies tested in a pre-clinical setting targeting TGFβ signaling, and some of these strategies are now in clinical trials, with models based on, for example, blockage of synthesis, ligand/receptor binding, antisense oligonucleotides and TGFβ antibodies. As with most anti-tumor strategies, however, one must bear in mind the suitability of the patient for the treatment, and how much each individual might benefit from a particular strategy.[40]

Seed: malignant melanoma cell; soil: lung.

The dense vascular surface of the lung makes this organ a suitable site for many primary tumor metastases, including melanoma, breast, bladder, colon, kidney, head and neck.[39] Lung metastatic lesions most commonly initiate at the level of small pulmonary arterioles. Here, they must either burst through or breach by another mechanism the endothelial junction of lung-blood vessels and the basement membrane underneath.[42]

Initially, studies involving experimental metastasis of malignant melanoma appeared to suggest that an upregulation of adhesion molecule expression (such as VCAM-1) was necessary in enhancing the number of successful metastases.[43] In contrast to this finding, it has more recently been established that there is no upregulation of VCAM-1 in spontaneous metastasis by melanomas, suggesting that this mechanism is not required for formation of metastasis.[44] There is, however, evidence to suggest that, once established, the organ-specific upregulation of VCAM-1 in the lungs as stimulated by metastatic tumor cells from the primary melanoma helps to support the growth of the metastases.[44]

Therefore, targeting strategies for VCAM-1 expression might be an attractive means to restrict growth and survival of metastatic tumors in the lung. Gosk et al.[45] propose one such targeting mechanism using immunoliposomes (IL) directed against VCAM-1. They demonstrate a selective in vivo targeting of tumor vessels versus non-affected organ vessels using these specific IL.

Seed: colorectal cancer cell; soil: liver.

The liver is one of the body's most densely vascularized tissues and its unique vascular supply via both the systemic circulation and the portal vein makes it a logical destination for circulating cancer cells. Gastrointestinal malignancies like colorectal carcinomas have a tendency to metastasize to the liver, likely due to the nature of the portal circulation. The vasculature in the liver is unique in comparison with other organs in that the sinusoids are highly permeable and do not represent a major barrier to extravasation.[4, 42]

It appears that the main barrier to the survival of circulating tumor cells in the liver is the ability to resist the immune response initiated by resident cells. Gene expression profiling of liver metastases from colorectal primary sites have revealed an upregulation of COX-2, an important player in immune regulation and angiogenesis.[46] Inhibition of COX-2 is now recognized as a potential preventative measure against development of liver metastasis based on studies using metastatic colorectal cancer cell lines.[47] The antineoplastic effects of COX-2 inhibition ex-vivo using tissue microarrays from liver metastases have also been demonstrated by assessing the number of apoptotic liver cells.[48]

In addition, it appears that some colorectal cancer cells have been found to express sialyl Lewis-X (sLex) and sialyl Lewis-A (sLea) antigens, and this correlates with both their prognosis and metastatic potential.[49, 50] Colorectal cancer cells support their adhesion to vascular endothelial cells in the liver through binding of the activated E-selectin on the vasculature to its ligands, sLex and sLea. It has also been shown that in studies with mice treated with E-selectin monoclonal antibodies, the treated mice form significantly less metastasis when sialyl Lewis expressing tumor cells are injected.[51] More recently, studies by Khatib et al.[52] have also supported the hypothesis that the inhibition of tumor-induced, hepatic microvessel E-selectin expression might provide a useful strategy for the prevention of hepatic metastasis based on mice studies in vivo.

Seed: small cell carcinoma (lung); soil: brain.

Between 20% and 40% of all cancer patients develop brain metastasis, with 30–50% of patients with lung cancer contributing greatly to this number. The colonization of the brain by a primary tumor results in an extremely poor prognosis for the affected individual, with the median survival time for patients with untreated brain metastasis being just 1–2 months.[53] The brain might be colonized in two ways, affecting either the brain parenchyma or the leptomeninges.[42]

Difficulties in colonizing the brain are mainly as a consequence of its lack of lymphatic drainage and the presence of the blood brain barrier (BBB), restricting even serum proteins unless they are shuttled across by active transport. This barrier limits and regulates molecular exchange at the interface between the blood and neural tissue or its fluid spaces.[54, 55]

This barrier must, therefore, be overcome by the invading tumor cells. However, mechanisms behind this in human brain metastasis are poorly understood. Focus instead has been on how the cells, once past the BBB, can thrive in the brain. Although glial cells and astrocytes can synthesize a large number of growth factors, cytokines and chemokines to support tumor cells, the metastatic tumor requires an increased vascular supply in order to maintain growth.[56]

Yano et al. show that highly brain metastatic cells produce a higher level of vascular endothelial growth factor (VEGF) protein than low brain metastatic cells in mice, confirmed both by mRNA expression analysis and immunohistochemistry. They find this expression to be necessary, but not sufficient for the angiogenesis and growth of brain metastasis.[57] The inhibition of VEGF in lung to brain metastasis, as in many other malignancies, is a promising target for an adjuvant treatment to radiotherapy. Bevacizumab, a monoclonal antibody for VEGFA has already been shown to have clinical activity in some metastatic cancers when combined with traditional cytotoxic therapy.[58-60]

Discussion

Stephen Paget concludes his seminal paper on metastasis in 1888 with the following words: “The best work in the pathology of cancer now is done by those who are studying the nature of the seed. They are like scientific botanists, and he who turns over the records of cases of cancer is only a ploughman, but his observations of the properties of the soil might also be useful.”

This is perhaps a slight understatement with regard to the importance of the soil in many metastases.

As we have seen, many of the mechanisms involved in a successful organ specific metastasis involve not only the specific properties of the metastatic cancer cell, but also an interaction between a specific property of the seed and one of the microenvironment in the soil. Indeed, when considering that some metastases arise up to 20 years after the removal of the primary tumor, tumor cell dormancy in metastatic sites appears to be controlled by a cue from the host tissue determining when the cell might begin to proliferate.

With regard to therapeutic implications, targeting metastatic progression is no mean feat. Table 3 summarizes a small number of current therapeutic targets and therapies, but there are many more. The lack of understanding in progression of primary tumor cells to metastatic cells and whether this occurs early or late in cancer development leads us to question when it is clinically relevant to administer anti-metastatic targets.[56] Recent studies have indicated that it might even be relevant to locally target the primary tumor in order to retard metastatic progression.[61] Compounds designed to interrupt metastatic development have not been generally shown to have sufficient efficacy in a clinical setting.

Table 3. Strategies for development of therapy targeting metastatic cancers
Primary tumorOrgan of metastasisTarget for therapyMechanism(s) targetedStatus
  1. CRC, colorectal cancer cell; VEGF, vascular endothelial growth factor.

Breast carcinomaBoneCXCR4/CXCL12 interaction, TGFβ expressionChemotaxis, angiogenesis, growthAnti CXCR4 and TGFβ antibodies in clinical trials
Malignant melanomaLungVCAM-1 expressionAdhesionMethods to downregulate VCAM1 under development
Colorectal carcinomaLiverInhibition of COX-2 and E-selectinInflammationCOX-2 inhibitor celecoxib shown to help prevent CRC development, E-selectin inhibitor cimetidine shows modest beneficial effect in CRC
Small cell carcinomaBrainInhibition of VEGFAngiogenesisVEGF-A inhibitor bevacizumab currently used for treatment of both lung and brain carcinoma

In conclusion, an increase in our understanding of mechanisms of invasion, tumor cell and stromal interactions has promoted another perspective on Paget's original hypothesis. The genetic background of the individual affecting the non-malignant cells of the host target organ has meant that the focus of the seed and soil hypothesis has shifted perspective from the seed being the most important factor, as suggested by Paget. It is now recognized that other components of the metastatic tumor's microenvironment are relevant. This increase in our knowledge provides a wealth of possible targets for therapy, many of which might contribute to prolonging life expectancy in patients with metastatic disease, provided they are administered to the right patient at an appropriate time.

Disclosure Statement

The authors have no conflict of interest.

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