These authors contributed equally to this work.
Comparative proteomic profiles indicating genetic factors may involve in hepatocellular carcinoma familial aggregation
Article first published online: 8 AUG 2012
© 2012 Japanese Cancer Association
Volume 103, Issue 10, pages 1833–1838, October 2012
How to Cite
(Cancer Sci, doi: 10.1111/j.1349-7006.2012.02368.x, 2012)
- Issue published online: 8 OCT 2012
- Article first published online: 8 AUG 2012
- Accepted manuscript online: 24 JUN 2012 10:25PM EST
- Manuscript Accepted: 14 JUN 2012
- Manuscript Revised: 3 JUN 2012
- Manuscript Received: 19 APR 2012
- National Natural Science Foundation. Grant Numbers: 30760219, 30960170
Familial aggregation of hepatocellular carcinoma (HCC), the third leading cause of cancer death worldwide, has shown to be a common phenomenon. We investigated the association between the genetic background and HCC familial aggregation. Serum samples were collected from HCC family members and normal control family members for screening the differentially expressed protein peaks with the approach of surface-enhanced laser desorption ionization time-of-flight mass spectrometry. Potential genetically associated protein peaks were selected and further identified by matrix assisted laser desorption ionization-time of flight mass spectrometry. A panel of six protein peaks (m/z 6432.94, 8478.35, 9381.91, 17284.67, 17418.34, and 18111.04) were speculated to reflect the genetic susceptibility of HCC familial aggregation. Three of them (m/z 6432.94, 8478.35, and 9381.91) were selected to identify as the candidate proteins. Nine identified proteins, including mostly apolipoprotein family (ApoA1, ApoA2, ApoC3, ApoE) and serum amyloid A protein (SAA), were found overexpressed in the multiple HCC cases family members. The comparative proteomic profiles have suggested that genetic factors ought to be taken into account for familial aggregation of HCC.