• Open Access

Prosaposin, a regulator of estrogen receptor alpha, promotes breast cancer growth

Authors

  • Yihong Wu,

    1. Department of Biochemistry and Molecular Biology, Shanghai Medical College of Fudan University, Shanghai, China
    2. Key Laboratory of Glycoconjugate Research, Ministry of Public Health, China
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  • Linlin Sun,

    1. Department of Biochemistry and Molecular Biology, Shanghai Medical College of Fudan University, Shanghai, China
    2. Key Laboratory of Glycoconjugate Research, Ministry of Public Health, China
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  • Weiying Zou,

    1. Department of Biochemistry and Molecular Biology, Shanghai Medical College of Fudan University, Shanghai, China
    2. Key Laboratory of Glycoconjugate Research, Ministry of Public Health, China
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  • Jiejie Xu,

    1. Department of Biochemistry and Molecular Biology, Shanghai Medical College of Fudan University, Shanghai, China
    2. Key Laboratory of Glycoconjugate Research, Ministry of Public Health, China
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  • Haiou Liu,

    1. Department of Biochemistry and Molecular Biology, Shanghai Medical College of Fudan University, Shanghai, China
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  • Wenzhong Wang,

    1. Department of Biochemistry and Molecular Biology, Shanghai Medical College of Fudan University, Shanghai, China
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  • Xiaojing Yun,

    Corresponding author
    1. Key Laboratory of Glycoconjugate Research, Ministry of Public Health, China
    • Department of Biochemistry and Molecular Biology, Shanghai Medical College of Fudan University, Shanghai, China
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  • Jianxin Gu

    Corresponding author
    1. Key Laboratory of Glycoconjugate Research, Ministry of Public Health, China
    • Department of Biochemistry and Molecular Biology, Shanghai Medical College of Fudan University, Shanghai, China
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To whom correspondence should be addressed.

E-mails: xjyun@fudan.edu.cn; jxgu@shmu.edu.cn

Abstract

Prosaposin, a secreted protein, is a well-known pleiotropic growth factor. Although a previous report has indicated that prosaposin is overexpressed in breast cancer cell lines, the role of prosaposin in the development of breast cancer remains to be identified. Here, we first revealed that prosaposin upregulated estrogen receptor alpha expression, nuclear translocation and transcriptional activity by western blot, immunofluorescence assay and dual luciferase reporter gene assay, respectively. Furthermore, we demonstrated prosaposin upregulated estrogen receptor alpha expression through MAPK-signaling pathway using MAPK inhibitor. Proliferation assay and tumor xenograft experiments in nude mice (n = 6 per group) further confirmed prosaposin could promote breast cancer growth significantly in vitro and in vivo. These findings suggested that prosaposin might enhance estrogen receptor alpha-mediated signaling axis and play a role in breast cancer development and progression.

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