Original Article

Overexpression of TRIM44 contributes to malignant outcome in gastric carcinoma

  1. Kingo Kashimoto1,†,
  2. Shuhei Komatsu1,†,*,
  3. Daisuke Ichikawa1,
  4. Tomohiro Arita1,
  5. Hirotaka Konishi1,
  6. Hiroaki Nagata1,
  7. Hiroki Takeshita1,
  8. Yukihisa Nishimura1,
  9. Shoji Hirajima1,
  10. Tsutomu Kawaguchi1,
  11. Atsushi Shiozaki1,
  12. Hitoshi Fujiwara1,
  13. Kazuma Okamoto1,
  14. Hitoshi Tsuda2,
  15. Eigo Otsuji1

Article first published online: 14 SEP 2012

DOI: 10.1111/j.1349-7006.2012.02407.x

Issue

Cancer Science

Cancer Science

Volume 103, Issue 11, pages 2021–2026, November 2012

Additional Information

How to Cite

(Cancer Sci 2012; 103: 2021–2026)

Author Information

  1. 1

    Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan

  2. 2

    Department of Pathology, National Cancer Center Hospital, Tokyo, Japan

  1. †

    These authors contributed equally to this work.

* To whom correspondence should be addressed.
E-mail: skomatsu@koto.kpu-m.ac.jp

  1. †

    These authors contributed equally to this work.

Publication History

  1. Issue published online: 29 OCT 2012
  2. Article first published online: 14 SEP 2012
  3. Accepted manuscript online: 6 AUG 2012 01:10AM EST
  4. Manuscript Accepted: 31 JUL 2012
  5. Manuscript Revised: 30 JUL 2012
  6. Manuscript Received: 5 APR 2012

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FilenameFormatSizeDescription
cas2407-sup-0001-FigureS1.tiffTIFF image1521KFig. S1. Positive and negative control of tripartite motif-containing protein 44 (TRIM44) immunostaining using formalin-fixed esophageal TE13 cell line.
cas2407-sup-0002-FigureS2.tiffTIFF image1521KFig. S2. Cell proliferation assay by downregulation of tripartite motif-containing protein 44 (TRIM44) expression in MKN45. Loss-of-function screening was done using siRNAs targeting TRIM44 in the MKN45 cell. The knockdown of a target gene was confirmed by Western blot analysis (left). For measurements of cell growth, the numbers of viable cells at various time points after transfection were assessed by the colorimetric water-soluble tetrazolium salt assay (right).
cas2407-sup-0003-FigureS3.tiffTIFF image1521KFig. S3. Patterns of immunohistochemistry according to the differentiation of gastric cancer.
cas2407-sup-0004-TableS1.tiffTIFF image1521KTable S1. Distribution of patients with tripartite motif-containing protein 44 (TRIM44) immunoreactivity in tumor cells according to the extent of intensity and proportion.

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