Association of alcohol dehydrogenase polymorphisms and life-style factors with excessive alcohol intake within the Spanish population (EPIC-Spain)
Article first published online: 20 AUG 2012
© 2012 The Authors, Addiction © 2012 Society for the Study of Addiction
Volume 107, Issue 12, pages 2117–2127, December 2012
How to Cite
Muñoz, X., Amiano, P., Celorrio, D., Dorronsoro, M., Sánchez, M.-J., Huerta, J. M., Barricarte, A., Arriola, L., Navarro, C., Molina-Montes, E., Chirlaque, M. D., Ardanaz, E., Rodriguez, L., Duell, E. J., Hijona, E., Herreros-Villanueva, M., Sala, N. and Bujanda, L. (2012), Association of alcohol dehydrogenase polymorphisms and life-style factors with excessive alcohol intake within the Spanish population (EPIC-Spain). Addiction, 107: 2117–2127. doi: 10.1111/j.1360-0443.2012.03970.x
- Issue published online: 20 NOV 2012
- Article first published online: 20 AUG 2012
- Accepted manuscript online: 12 JUN 2012 06:31AM EST
- Manuscript Accepted: 29 MAY 2012
- Manuscript Revised: 20 MAR 2012
- Manuscript Received: 8 FEB 2012
- Spanish Ministry of Health and Consumer Affairs
- Carlos III Health Institute (ISCIII). Grant Number: 2007-047O
- ADH6 ;
- ADH1B ;
- excessive alcohol intake;
- genetic susceptibility;
- life-style factors;
To analyse associations between alcohol dehydrogenase (ADH) polymorphisms and alcohol intake in Spanish men and women.
Design and settings
We analysed the relationship between 21 genetic variants in ADH genes and excessive alcohol intake in both men and women. Single nucleotide polymorphisms (SNPs) were genotyped using a customized array and a sex-stratified analysis was performed.
Ethanol intake was calculated using a validated dietary history questionnaire.
Heavy consumers of alcohol (≥70 g/day in men, ≥42 g/day in women) (653 cases) and very low or non-consumers (<2 g/day) (880 controls) from the Spanish cohort of the European Prospective Investigation into Cancer (EPIC).
We found statistically significant associations between alcohol intake and known life-style factors; namely, smoking and food energy intake (meat and fruit/seeds) in both men and women, as well as with physical activity in women and educational level in men. Additionally, we found that a non-synonymous coding SNP in ADH1B (rs1229984) is associated inversely with excessive alcohol intake in men [odds ratio (OR) = 0.19, 95% confidence interval (CI) = 0.11–0.33; P = 4.77E−10) and women (OR = 0.48, 95% CI = 0.27–0.83; P = 0.0067). Furthermore, ADH6 rs3857224 was found associated with heavy alcohol intake in women (OR = 1.61, 95% CI = 1.21–2.14; P = 1.01E−3), but not in men.
In the Spanish population, the single nucleotide polymorphism of alcohol dehydrogenase ADH1B, rs1229984, is associated inversely with alcohol intake in both men and women. Another polymorphism of ADH6, rs3857224, is associated with heavy alcohol intake in women.