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The tobacco Ntann12 gene, encoding an annexin, is induced upon Rhodoccocus fascians infection and during leafy gall development

Authors

  • OLIVIER VANDEPUTTE,

    1. Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, Rue Adrienne Bolland 8, B-6041 Gosselies, Belgium
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  • YVES OUKOUOMI LOWE,

    1. Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, Rue Adrienne Bolland 8, B-6041 Gosselies, Belgium
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  • SYLVIA BURSSENS,

    1. The Institute of Plant Biotechnology for Developing Countries, Department of Molecular Genetics, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium
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  • DAMIEN VAN RAEMDONCK,

    1. Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, Rue Adrienne Bolland 8, B-6041 Gosselies, Belgium
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  • DAVID HUTIN,

    1. Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, Rue Adrienne Bolland 8, B-6041 Gosselies, Belgium
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  • DELPHINE BONIVER,

    1. Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, Rue Adrienne Bolland 8, B-6041 Gosselies, Belgium
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  • DANNY GEELEN,

    1. Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, Technologie Park 927, B-9052 Gent, Belgium
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  • MONDHER EL JAZIRI,

    1. Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, Rue Adrienne Bolland 8, B-6041 Gosselies, Belgium
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  • MARIE BAUCHER

    Corresponding author
    1. Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, Rue Adrienne Bolland 8, B-6041 Gosselies, Belgium
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* Correspondence: Tel.: +32 26509579; Fax: +32 26509578; E-mail: mbaucher@ulb.ac.be

SUMMARY

Annexins are calcium-binding proteins that have been associated in plants with different biological processes such as responses to abiotic stress and early nodulation stages. Until now, the implication of annexins during plant–pathogen interactions has not been reported. Here, a novel plant annexin gene induced in tobacco BY-2 cell suspension cultures infected with the phytopathogenic bacterium Rhodococcus fascians (strain D188) has been identified. Expression of this gene, called Ntann12, is also induced, but to a lower extent, by a strain (D188-5) that is unable to induce leafy gall formation. This gene was also induced in BY-2 cells infected with Pseudomonas syringae but not in cells infected with Agrobacterium tumefaciens or Escherichia coli. Ntann12 expression was also found to be stimulated by abiotic stress, including NaCl and abscissic acid, confirming a putative role in stress signal transduction pathways. In addition, promoter-GUS analyses using homozygous transgenic tobacco seedlings showed that the developmentally controlled expression of Ntann12 is altered upon R. fascians infection. Finally, up-regulation of Ntann12 during leafy gall ontogenesis was confirmed by RT-qPCR. Discussion is focused on the potential role of Ntann12 in biotic and abiotic stress responses and in plant development, both processes that may involve Ca2+-dependent signalling.

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