SEARCH

SEARCH BY CITATION

Fig. S1 The Xanthomonas campestris pv. campestris type III secretion system (Xcc TTSS) is required for callose induction. Arabidopsis leaves were infiltrated with the indicated bacterial strains at 2 × 107 colony-forming units (CFU)/mL, and callose deposition was examined 24 h later. Average numbers of callose deposits per microscopic field of 0.1 mm2 are shown below each photograph. ‘±’ represents the standard deviations from three replicates. Similar results were obtained in three independent experiments.

Fig. S2 Type III secretion system (TTSS) mutants ΔhrcV, ΔhrpG and ΔhrpX are nonpathogenic on Arabidopsis. Five-week-old Col-0 plants were inoculated with wild-type (WT) Xanthomonas campestris pv. campestris strain 8004 (Xcc 8004), ΔhrcV, ΔhrpG and ΔhrpX mutants [bacterial suspension at a concentration of 5 × 105 colony-forming units (CFU)/mL]. The bacterial populations were determined at 0 and 3 days post-inoculation. Each data point represents three replicates. Error bars indicate standard deviations. The experiments were repeated twice with similar results.

Fig. S3 Electrolyte leakage assay on leaves infiltrated with wild-type (WT) Xanthomonas campestris pv. campestris strain 8004 (Xcc 8004) or ΔhrcV mutant. Five-week-old Arabidopsis leaves were inoculated with DC3000 (avrB), WT Xcc 8004 and ΔhrcV mutant [bacterial suspension at a concentration of 2 × 107 colony-forming units (CFU)/mL]. The electrolyte leakage was measured at the indicated times. Each data point represents three replicates. Error bars indicate standard deviations. The experiments were repeated twice with similar results.

Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.

FilenameFormatSizeDescription
MPP_642_sm_f1.jpg116KSupporting info item
MPP_642_sm_f2.jpg101KSupporting info item
MPP_642_sm_f3.jpg69KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.