CorA, the magnesium/nickel/cobalt transporter, affects virulence and extracellular enzyme production in the soft rot pathogen Pectobacterium carotovorum

Authors

  • CALEB M. KERSEY,

    1. Department of Agricultural Sciences, Tennessee State University, 3500 John A. Merritt Blvd, Campus Box 9610, Nashville, TN 37209, USA
    2. Department of Biological Sciences, Tennessee State University, 3500 John A. Merritt Blvd, Campus Box 9610, Nashville, TN 37209, USA
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  • PAUL A. AGYEMANG,

    1. Department of Agricultural Sciences, Tennessee State University, 3500 John A. Merritt Blvd, Campus Box 9610, Nashville, TN 37209, USA
    2. Department of Biological Sciences, Tennessee State University, 3500 John A. Merritt Blvd, Campus Box 9610, Nashville, TN 37209, USA
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  • C. KORSI DUMENYO

    Corresponding author
    1. Department of Agricultural Sciences, Tennessee State University, 3500 John A. Merritt Blvd, Campus Box 9610, Nashville, TN 37209, USA
    2. Department of Biological Sciences, Tennessee State University, 3500 John A. Merritt Blvd, Campus Box 9610, Nashville, TN 37209, USA
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Errata

This article is corrected by:

  1. Errata: CorA, the magnesium/nickel/cobalt transporter, affects virulence and extracellular enzyme production in the soft rot pathogen Pectobacterium carotovorum Volume 13, Issue 3, 327, Article first published online: 28 February 2012

Email: cdumenyo@tnstate.edu

SUMMARY

Pectobacterium carotovorum (formerly Erwinia carotovora ssp. carotovora) is a phytopathogenic bacterium that causes soft rot disease, characterized by water-soaked soft decay, resulting from the action of cell wall-degrading exoenzymes secreted by the pathogen. Virulence in soft rot bacteria is regulated by environmental factors, host and bacterial chemical signals, and a network of global and gene-specific bacterial regulators. We isolated a mini-Tn5 mutant of P. carotovorum that is reduced in the production of extracellular pectate lyase, protease, polygalacturonase and cellulase. The mutant is also decreased in virulence as it macerates less host tissues than its parent and is severely impaired in multiplication in planta. The inactivated gene responsible for the reduced virulent phenotype was identified as corA. CorA, a magnesium/nickel/cobalt membrane transporter, is the primary magnesium transporter for many bacteria. Compared with the parent, the CorA- mutant is cobalt resistant. The mutant phenotype was confirmed in parental strain P. carotovorum by marker exchange inactivation of corA. A functional corA+ DNA from P. carotovorum restored exoenzyme production and pathogenicity to the mutants. The P. carotovorum corA+ clone also restored motility and cobalt sensitivity to a CorA- mutant of Salmonella enterica. These data indicate that CorA is required for exoenzyme production and virulence in P. carotovorum.

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