Characterization of polygenic resistance to powdery mildew in tomato at cytological, biochemical and gene expression level

Authors

  • CHENGWEI LI,

    1. Wageningen UR Plant Breeding, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708PB Wageningen, the Netherlands
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    • Present address: Key Laboratory of Genetics and Molecular Breeding, Department of Life Science, Zhoukou Normal University, Zhoukou 466001, China.

    • These authors contributed equally to this work.

  • LUIGI FAINO,

    1. Wageningen UR Plant Breeding, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708PB Wageningen, the Netherlands
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    • These authors contributed equally to this work.

  • LIN DONG,

    1. Wageningen UR Plant Breeding, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708PB Wageningen, the Netherlands
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  • JUNMEI FAN,

    1. Wageningen UR Plant Breeding, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708PB Wageningen, the Netherlands
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  • LEVENTE KISS,

    1. Plant Protection Institute of the Hungarian Academy of Sciences, PO Box 102, H-1525 Budapest, Hungary
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  • CLAUDIO DE GIOVANNI,

    1. Department of Agroforestry, Environmental Biology and Chemistry, Section of Genetics and Plant Breeding, University of Bari, Via Amendola 165/A, 70126 Bari, Italy
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  • ALES LEBEDA,

    1. Palacký University in Olomouc, Faculty of Science, Department of Botany, Šlechtitelů 11, Olomouc-Holice, Czech Republic
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  • JOHN SCOTT,

    1. Gulf Coast Research and Education Center, University of Florida, 14625 CR 672, Wimauma, FL 33598, USA
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  • YOSHINORI MATSUDA,

    1. Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University, Nara 631-8505, Japan
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  • HIDEYOSHI TOYODA,

    1. Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University, Nara 631-8505, Japan
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  • PIM LINDHOUT,

    1. Wageningen UR Plant Breeding, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708PB Wageningen, the Netherlands
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  • RICHARD G. F. VISSER,

    1. Wageningen UR Plant Breeding, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708PB Wageningen, the Netherlands
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  • GUUSJE BONNEMA,

    1. Wageningen UR Plant Breeding, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708PB Wageningen, the Netherlands
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  • YULING BAI

    Corresponding author
    1. Wageningen UR Plant Breeding, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708PB Wageningen, the Netherlands
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: Email: bai.yuling@wur.nl

SUMMARY

Extensive research in the area of plant innate immunity has increased considerably our understanding of the molecular mechanisms associated with resistance controlled by a dominant resistance gene. In contrast, little is known about the molecular basis underlying the resistance conferred by quantitative trait loci (QTLs). In this study, using the interaction of tomato (Solanum lycopersicum) with Oidium neolycopersici, we compared the cytological, biochemical and molecular mechanisms involved in both monogenic and polygenic resistances conferred by a dominant gene (Ol-1) and three QTLs (Ol-qtls), respectively. Our results showed that the three Ol-qtls jointly confer a very high level of broad-spectrum resistance and that the resistance is associated with both the hypersensitive response and papillae formation, with the hypersensitive response being prevalent. Both H2O2 and callose accumulation, which are coupled with Ol-1-mediated resistance, are also associated with the resistance conferred by Ol-qtls. Further, we analysed the pathogen-induced transcript profiles of near-isogenic lines carrying the three Ol-qtls and the Ol-1 gene. Transcript profiles obtained by cDNA-amplified fragment length polymorphism analysis showed that, on fungal challenge, about 70% of the transcript-derived fragments are up-regulated in both susceptible and resistant genotypes. Most of the sequenced transcript-derived fragments showed homology to genes with functions in defence responses, suggesting that defence-responsive genes responsible for basal defence are involved in both monogenic and polygenic resistances conferred by Ol-1 and Ol-qtls, respectively. Although about 18% of the identified transcript-derived fragments are specific for either monogenic or polygenic resistance, their expression patterns need to be further verified by quantitative reverse transcriptase-polymerase chain reaction.

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