These authors contributed equally to this work.
Multilocus sequence analysis and type III effector repertoire mining provide new insights into the evolutionary history and virulence of Xanthomonas oryzae
Version of Record online: 19 SEP 2011
© 2011 THE AUTHORS. MOLECULAR PLANT PATHOLOGY © 2011 BSPP AND BLACKWELL PUBLISHING LTD
Molecular Plant Pathology
Volume 13, Issue 3, pages 288–302, April 2012
How to Cite
HAJRI, A., BRIN, C., ZHAO, S., DAVID, P., FENG, J.-X., KOEBNIK, R., SZUREK, B., VERDIER, V., BOUREAU, T. and POUSSIER, S. (2012), Multilocus sequence analysis and type III effector repertoire mining provide new insights into the evolutionary history and virulence of Xanthomonas oryzae. Molecular Plant Pathology, 13: 288–302. doi: 10.1111/j.1364-3703.2011.00745.x
- Issue online: 28 FEB 2012
- Version of Record online: 19 SEP 2011
Fig. S1 Plot of the Bayesian assignment of Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc) strains to clusters based on the analysis of the concatenated dataset (glnA, gyrB and rpoD sequences) using the no admixture model of structure 2.1 software. Each haplotype is represented by a line and the geographical origin of the strains is given.
Fig. S2 The type III effector (T3E) XopO is not essential for the virulence and tissue specificity of Xanthomonas oryzae in rice Nipponbare. The bacterial cells were suspended in sterilized water and the cell concentration was adjusted to an optical density at 600 nm (OD600) of 0.2 '2 × 108 colony-forming units (cfu)/mL'. Data shown are the means ± standard deviation from three replications, each with 30 leaves, from one representative experiment; similar results were obtained in the other two independent experiments. (a) Average length of lesions caused by Xanthomonas oryzae pv. oryzicola 7 days post-inoculation. Approximately 20 µL of bacterial culture suspension were infiltrated into leaf mesophyll tissue with a 1-mL blunt-end plastic syringe. (b) Average length of lesions caused by Xanthomonas oryzae pv. oryzae inoculated by the leaf-clipping method, 14 days after inoculation. (c) Average length of lesions caused by Xanthomonas oryzae pv. oryzae inoculated by the leaf infiltration method, 7 days after inoculation.
Table S1 The type III effector (T3E) genes analysed in this study: gene functions and primer sequences used for polymerase chain reaction (PCR) amplifications and for the preparation of probes for dot-blot hybridizations.
Table S2 Polymerase chain reaction (PCR) primers used for the functional analysis of the type III effector (T3E) gene xopO in Xanthomonas oryzae.
|MPP_745_sm_Fig_S1.jpg||104K||Supporting info item|
|MPP_745_sm_Fig_S2.rar||1029K||Supporting info item|
|MPP_745_sm_Table_S1.doc||72K||Supporting info item|
|MPP_745_sm_Table_S2.doc||27K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.