These authors contributed equally to this work.
TaWIR1 contributes to post-penetration resistance to Magnaporthe oryzae, but not Blumeria graminis f. sp. tritici, in wheat
Article first published online: 16 JAN 2012
© 2012 THE AUTHORS. MOLECULAR PLANT PATHOLOGY © 2012 BSPP AND BLACKWELL PUBLISHING LTD
Molecular Plant Pathology
Volume 13, Issue 7, pages 653–665, September 2012
How to Cite
TUFAN, H. A., MCGRANN, G. R. D., MACCORMACK, R. and BOYD, L. A. (2012), TaWIR1 contributes to post-penetration resistance to Magnaporthe oryzae, but not Blumeria graminis f. sp. tritici, in wheat. Molecular Plant Pathology, 13: 653–665. doi: 10.1111/j.1364-3703.2011.00775.x
- Issue published online: 26 JUL 2012
- Article first published online: 16 JAN 2012
Fig. S1 CLUSTALW nucleotide alignment of BSMV:WIR1 and TaWIR1 sequences showing conservation of the target region between TaWIR1 genes. Shading indicates 100% (black), 75% (dark grey) and 50% (light grey) conservation between sequences.
Fig. S2 Barley stripe mosaic virus (BSMV)-mediated virus-induced gene silencing (VIGS) of TaWIR1 reduces the expression of transcripts corresponding to probe sets Ta.21556.1, Ta.22732.1 and Ta.3133.1 on the Affymetrix Wheat GeneChip, despite these sequence having less than 80% similarity to BSMV:WIR1. Transcript levels were measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and shown relative to mock-inoculated controls. The mean values of three independent experiments are shown with standard error bars.
Fig. S3 Transcript levels of TaWIR1 genes in intact leaves of cv. Renan measured after inoculation (hours post-inoculation, hpi) with Magnaporthe oryzae isolate BR32, relative to mock-inoculated controls. The mean values of two independent experiments are shown with standard error bars.
Fig. S4 Transcript levels of TaWIR1 genes in cv. Renan leaf tissues inoculated with Magnaporthe oryzae isolate BR32, relative to the same region of the leaf in the mock-inoculated control. The mean values of three independent experiments are shown with standard error bars.
Table S1 TaWIR1 transcripts present as probe sets on the Affymetrix Wheat GeneChip.
Table S2 Primer sequences.
Table S3 TaWIR1 genes identified from database similarity searches.
Table S4 SignalP analysis of TaWIR1 proteins.
Table S5 TargetP analysis of TaWIR1 proteins.
Table S6 Transmembrane prediction for TaWIR1 proteins.
Table S7 Nucleotide identities of TaWIR1 sequences to BSMV:WIR1.
Table S8 BSMV:WIR1 silencing targets predicted by si-Fi analysis.
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