Get access

MoSwi6, an APSES family transcription factor, interacts with MoMps1 and is required for hyphal and conidial morphogenesis, appressorial function and pathogenicity of Magnaporthe oryzae

Authors

  • ZHONGQIANG QI,

    1. Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China
    Search for more papers by this author
    • These authors contributed equally to this work.

  • QI WANG,

    1. Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China
    Search for more papers by this author
    • These authors contributed equally to this work.

  • XIANYING DOU,

    1. Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China
    Search for more papers by this author
  • WEI WANG,

    1. Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China
    Search for more papers by this author
  • QIAN ZHAO,

    1. Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China
    Search for more papers by this author
  • RUILI LV,

    1. Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China
    Search for more papers by this author
  • HAIFENG ZHANG,

    1. Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China
    Search for more papers by this author
  • XIAOBO ZHENG,

    1. Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China
    Search for more papers by this author
  • PING WANG,

    1. The Research Institute for Children and Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans, LA 70118, USA
    Search for more papers by this author
  • ZHENGGUANG ZHANG

    Corresponding author
    1. Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China
    Search for more papers by this author

: Email: zhgzhang@njau.edu.cn

SUMMARY

The Magnaporthe oryzae mitogen-activated protein kinase (MAPK) MoMps1 plays a critical role in the regulation of various developmental processes, including cell wall integrity, stress responses and pathogenicity. To identify potential effectors of MoMps1, we characterized the function of MoSwi6, a homologue of Saccharomyces cerevisiae Swi6 downstream of MAPK Slt2 signalling. MoSwi6 interacted with MoMps1 both in vivo and in vitro, suggesting a possible functional link analogous to Swi6–Slt2 in S. cerevisiae. Targeted gene disruption of MoSWI6 resulted in multiple developmental defects, including reduced hyphal growth, abnormal formation of conidia and appressoria, and impaired appressorium function. The reduction in appressorial turgor pressure also contributed to an attenuation of pathogenicity. The ΔMoswi6 mutant also displayed a defect in cell wall integrity, was hypersensitive to oxidative stress, and showed a significant reduction in transcription and activity of extracellular enzymes, including peroxidases and laccases. Collectively, these roles are similar to those of MoMps1, confirming that MoSwi6 functions in the MoMps1 pathway to govern growth, development and full pathogenicity.

Ancillary