These authors contributed equally to this work.
Possible involvement of maize Rop1 in the defence responses of plants to viral infection
Article first published online: 14 FEB 2012
© 2012 THE AUTHORS. MOLECULAR PLANT PATHOLOGY © 2012 BSPP AND BLACKWELL PUBLISHING LTD
Molecular Plant Pathology
Volume 13, Issue 7, pages 732–743, September 2012
How to Cite
CAO, Y., SHI, Y., LI, Y., CHENG, Y., ZHOU, T. and FAN, Z. (2012), Possible involvement of maize Rop1 in the defence responses of plants to viral infection. Molecular Plant Pathology, 13: 732–743. doi: 10.1111/j.1364-3703.2011.00782.x
- Issue published online: 26 JUL 2012
- Article first published online: 14 FEB 2012
The expression of host genes can be altered during the process of viral infection. To investigate the viral infection-induced up-regulated gene expression changes of maize at different time intervals post-inoculation with Sugarcane mosaic virus (SCMV), a suppression subtractive hybridization cDNA library was constructed. A total of 454 cDNA clones were identified to be viral infection-induced up-regulated genes. The influence of Rop1 on the infection of maize by SCMV was investigated. The results showed that transient silencing of the ZmRop1 gene through virus-induced gene silencing enhanced the accumulation and systemic infection of SCMV and another potyvirus (Pennisetum mosaic virus) in maize plants, whereas transient over-expression of ZmRop1 in maize protoplasts reduced SCMV accumulation. Furthermore, it was demonstrated that the heterologous expression of ZmRop1 impaired Potato virus X infection in Nicotiana benthamiana plants. These data suggest that ZmRop1 may play a role in plant defence responses to viral infection.